miR-223 Inhibits Lipid Deposition and Inflammation by Suppressing Toll-Like Receptor 4 Signaling in Macrophages
Abstract
:1. Introduction
2. Results
2.1. Expression of miR-223 Is Up-Regulated in Atherosclerotic Lesions in ApoE−/− Mice
2.2. Down-Regulation of miR-223 Was Confirmed in Macrophages Stimulated with TLR Agonists
2.3. miR-223 Attenuated LPS-Triggered Foam Cell Formation
2.4. miR-223 Negatively Regulated Inflammatory Response to LPS in TLR-Triggered Macrophages
2.5. Activation of the TLR4-NF-κB Pathway Is Abrogated by miR-223 Mimics and Responsible for the Inhibitory Effect of miR-223 on Lipid Accumulation and Inflammatory Response
2.6. miR-223 Mitigates TLR4 Signaling Activation through the PI3K/AKT Pathway
3. Discussion
4. Experimental Section
4.1. Reagents and Antibodies
4.2. Animal Model of Atherosclerosis
4.3. Macrophage Cell Culture and Treatment
4.4. Oligonucleotide Transfection
4.5. RNA Extraction and Quantitative RT-PCR
4.6. Oil Red O Staining
4.7. Lipid Assay by High-Performance Liquid Chromatography (HPLC)
4.8. Cholesterol Efflux Analysis
4.9. Measurement of IL-6 Cytokine Production
4.10. Determination of NO Concentration
4.11. Western Blotting Assay
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Conflicts of Interest
References
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Wang, J.; Bai, X.; Song, Q.; Fan, F.; Hu, Z.; Cheng, G.; Zhang, Y. miR-223 Inhibits Lipid Deposition and Inflammation by Suppressing Toll-Like Receptor 4 Signaling in Macrophages. Int. J. Mol. Sci. 2015, 16, 24965-24982. https://doi.org/10.3390/ijms161024965
Wang J, Bai X, Song Q, Fan F, Hu Z, Cheng G, Zhang Y. miR-223 Inhibits Lipid Deposition and Inflammation by Suppressing Toll-Like Receptor 4 Signaling in Macrophages. International Journal of Molecular Sciences. 2015; 16(10):24965-24982. https://doi.org/10.3390/ijms161024965
Chicago/Turabian StyleWang, Jun, Xiaojun Bai, Qiang Song, Fenling Fan, Zhi Hu, Gesheng Cheng, and Yushun Zhang. 2015. "miR-223 Inhibits Lipid Deposition and Inflammation by Suppressing Toll-Like Receptor 4 Signaling in Macrophages" International Journal of Molecular Sciences 16, no. 10: 24965-24982. https://doi.org/10.3390/ijms161024965