Interferon α Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced Pathway
Abstract
:1. Introduction
2. Results
2.1. Interferon α (IFN-α) Inhibits the Proliferation of HeLa Cells
2.2. IFN-α Induces the Apoptosis of HeLa Cells
2.3. The Mitochondrial Apoptotic Pathway Is Activated by IFN-α Treatment
2.4. IFN-α Activates the ER Stress-Induced Apoptotic Pathway but Not the Extrinsic Apoptotic Pathway in HeLa Cells
2.5. The ER Stress-Induced Apoptotic Pathway and the Mitochondrial Apoptotic Pathway Are Independently Induced by IFN-α
3. Discussion
4. Materials and Methods
4.1. Cell Lines, Cell Culture, Chemicals, and Reagents
4.2. Glucose/Lactate Assay
4.3. Cell Proliferation Assay
4.4. Flow Cytometric Analysis
4.5. Mitochondria Isolation
4.6. Real-Time Quantatitive Reverse Transcription PCR (qRT-PCR)
4.7. Western Blot Analysis
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gene Name | GenBank ID | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | Size of Product (bp) |
---|---|---|---|---|
β-Actin | BC009275 | TCCATCATGAAGTGTGACGT | CTCAGGAGGAGCAATGATCT | 161 |
Caspase 4 | EF636667 | TTGCTTTCTGCTCTTCAACG | GTGTGATGAAGATAGAGCCCATT | 72 |
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Shi, W.-Y.; Cao, C.; Liu, L. Interferon α Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced Pathway. Int. J. Mol. Sci. 2016, 17, 1832. https://doi.org/10.3390/ijms17111832
Shi W-Y, Cao C, Liu L. Interferon α Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced Pathway. International Journal of Molecular Sciences. 2016; 17(11):1832. https://doi.org/10.3390/ijms17111832
Chicago/Turabian StyleShi, Wei-Ye, Cheng Cao, and Li Liu. 2016. "Interferon α Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced Pathway" International Journal of Molecular Sciences 17, no. 11: 1832. https://doi.org/10.3390/ijms17111832