Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of the Stipa purpurea SpCIPK26 Gene
2.2. Overexpression of SpCIPK26 Enhances Tolerance to Drought and Salt Stress in Arabidopsis thaliana
2.3. Overexpression of SpCIPK26 Increases Water Potential and Abscisic Acid Content and Decreases Reactive Oxygen Species Accumulation
2.4. The Abscisic Acid-Dependent Signaling Pathway Contributes to the Drought and Salt Tolerance of SpCIPK26-Overexpressing Plants
2.5. Upregulation of Stress-Related Genes of SpCIPK26-OXP Plants in Response to Abscisic Acid, Drought, and Salt Treatments
3. Discussion
4. Materials and Methods
4.1. Growth Conditions and Stress Treatments
4.2. SpCIPK26 Cloning and Sequence Analysis
4.3. SpCIPK26 Expression Vector for Arabidopsis thaliana Transformation
4.4. Germination and Phenotype Assays
4.5. Quantitative Reverse Transcription Polymerase Chain Reaction
4.6. Fv/Fm and Water Potential
4.7. Abscisic Acid Contents
4.8. In Situ Reactive Oxygen Species Detection
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
CIPK | Calcineurin B-Like Protein-Interacting Protein Kinase |
ROS | Reactive Oxygen Species |
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Zhou, Y.; Sun, X.; Yang, Y.; Li, X.; Cheng, Y.; Yang, Y. Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling. Int. J. Mol. Sci. 2016, 17, 966. https://doi.org/10.3390/ijms17060966
Zhou Y, Sun X, Yang Y, Li X, Cheng Y, Yang Y. Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling. International Journal of Molecular Sciences. 2016; 17(6):966. https://doi.org/10.3390/ijms17060966
Chicago/Turabian StyleZhou, Yanli, Xudong Sun, Yunqiang Yang, Xiong Li, Ying Cheng, and Yongping Yang. 2016. "Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling" International Journal of Molecular Sciences 17, no. 6: 966. https://doi.org/10.3390/ijms17060966