H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity
Abstract
:1. Introduction
2. Results
2.1. Effects of Cu and Cd on H2O2 Production in Rice Radicles
2.2. Cu and Cd Up-Regulate the ricMT and CuZn-SOD Gene Expression in Rice Radicles
2.3. ricMT Expression Improved Cu and Cd Tolerance of Rice Suspension Cells
2.4. ricMT Expression Decreased H2O2 Production in Rice Suspension Cells under Cu and Cd Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Generation of Sense and Antisense ricMT Transgenic Rice
4.3. Suspension Cell Cultures
4.4. Total RNA Isolation, cDNA Synthesis and Quantitative RT-PCR
4.5. Hydrogen Peroxide Localization In Situ
4.6. H2O2 Determination in Extracts
4.7. Evans Blue Assay for Suspension Cell Death
4.8. Statistical Analysis
Acknowledgments
Author Contributions
Abbreviations
Cys | cysteine |
ROS | reactive oxygen species |
H2DCFDA | 2′,7′-dichlorodihydrofluorescein diacetate |
MT | metallothionein |
SOD | superoxide dismutase |
WT | wild-type |
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Zhang, H.; Lv, S.; Xu, H.; Hou, D.; Li, Y.; Wang, F. H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity. Int. J. Mol. Sci. 2017, 18, 2083. https://doi.org/10.3390/ijms18102083
Zhang H, Lv S, Xu H, Hou D, Li Y, Wang F. H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity. International Journal of Molecular Sciences. 2017; 18(10):2083. https://doi.org/10.3390/ijms18102083
Chicago/Turabian StyleZhang, Hongxiao, Shufang Lv, Huawei Xu, Dianyun Hou, Youjun Li, and Fayuan Wang. 2017. "H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity" International Journal of Molecular Sciences 18, no. 10: 2083. https://doi.org/10.3390/ijms18102083