Aquaporin Expression and Water Transport Pathways inside Leaves Are Affected by Nitrogen Supply through Transpiration in Rice Plants
Abstract
:1. Introduction
2. Results
2.1. Gas-Exchange Was Affected by N Supply
2.2. Aquaporin Expression Was Affected by N Supply
2.3. Hydraulic Conductance Was Affected by N Supply
2.4. The Relationship between Aquaporin Expression, Transpiration and Cell Wall Thickness
2.5. The Relationship between Transpiration Rate and Intercellular Air Space (fIAS)
3. Discussion
3.1. N Supply Increase Photosynthetic CO2 Fixation
3.2. N Supply Affecting Aquaporin Expression and Hydraulic Conductance
3.3. The Interaction between Transpiration and Aquaporin Expression
4. Materials and Methods
4.1. Plant Materials
4.2. Gas Exchange Measurement of Newly Expanded Leaves
4.3. Leaf Hydraulic Conductance (Kleaf) Mesurement of Newly Expanded Leaves
4.4. Real-Time-Quantitative PCR (RT-qPCR)
4.5. Electron Microscopy
4.6. Statistical Analysis
5. Conclusions and Perspectives
Supplementary Materials
Acknowledgment
Author Contribution
Conflicts of Interest
References
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Ding, L.; Li, Y.; Gao, L.; Lu, Z.; Wang, M.; Ling, N.; Shen, Q.; Guo, S. Aquaporin Expression and Water Transport Pathways inside Leaves Are Affected by Nitrogen Supply through Transpiration in Rice Plants. Int. J. Mol. Sci. 2018, 19, 256. https://doi.org/10.3390/ijms19010256
Ding L, Li Y, Gao L, Lu Z, Wang M, Ling N, Shen Q, Guo S. Aquaporin Expression and Water Transport Pathways inside Leaves Are Affected by Nitrogen Supply through Transpiration in Rice Plants. International Journal of Molecular Sciences. 2018; 19(1):256. https://doi.org/10.3390/ijms19010256
Chicago/Turabian StyleDing, Lei, Yingrui Li, Limin Gao, Zhifeng Lu, Min Wang, Ning Ling, Qirong Shen, and Shiwei Guo. 2018. "Aquaporin Expression and Water Transport Pathways inside Leaves Are Affected by Nitrogen Supply through Transpiration in Rice Plants" International Journal of Molecular Sciences 19, no. 1: 256. https://doi.org/10.3390/ijms19010256