Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.)
Abstract
:1. Introduction
2. Results
2.1. Identification of the TaHAKs in Wheat
2.2. Phylogenetic Analysis of TaHAK Proteins
2.3. Genomic Distribution of TaHAKs
2.4. Gene Structure and Conserved Motif Analyses of TaHAKs
2.5. Expression Analysis of TaHAK Genes in Various Wheat Tissues
2.6. Expression Analysis of TaHAK Genes under Various Stresses
2.7. TaHAK1b-2BL Functions in Potassium Uptake and Salt Tolerance in Yeast
3. Discussion
4. Materials and Methods
4.1. Identification of TaHAK Genes in Wheat
4.2. Phylogenetic Analysis of TaHAKs
4.3. Physical Localization of TaHAK Genes
4.4. Analysis of Gene Structures and Protein Motifs
4.5. Plant Materials, Growth Conditions, and Experimental Treatments
4.6. RNA Isolation and qRT-PCR
4.7. Subcellular Location of the TaHAK1b-2BL Protein
4.8. Functional Complementation Assay of TaHAK1b-2BL in Yeast
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene Name | Ensembl ID | Amino Acid Length | TMS | Intron No. | Exon No. | S.L. | Gene Location (bp) | |
---|---|---|---|---|---|---|---|---|
Start | End | |||||||
TaHAK1a-4BL | TRIAE_CS42_4BL_TGACv1_321685_AA1064100.1 | 784 | 11 | 7 | 8 | PM | 600657723 | 600654569 |
TaHAK1a-4DL | TRIAE_CS42_4DL_TGACv1_343636_AA1137540.1 | 778 | 11 | 8 | 9 | PM | 476306655 | 476302887 |
TaHAK1b-2BL | TRIAE_CS42_2BL_TGACv1_129472_AA0385230.2 | 768 | 11 | 8 | 9 | PM | 436243088 | 436237762 |
TaHAK1b-2DL | TRIAE_CS42_2DL_TGACv1_158352_AA0516430.1 | 776 | 11 | 8 | 9 | PM | 367507729 | 367502225 |
TaHAK1b-2A | TRIAE_CS42_U_TGACv1_641460_AA2095620.1 | 772 | 11 | 8 | 9 | PM | 497657505 | 497652193 |
TaHAK1c-2BL | TRIAE_CS42_2BL_TGACv1_129437_AA0383630.1 | 774 | 10 | 8 | 9 | PM | 436956667 | 436962029 |
TaHAK2-3AL | TRIAE_CS42_3AL_TGACv1_194420_AA0632700.2 | 784 | 13 | 8 | 9 | PM | 695496006 | 695500670 |
TaHAK2-3DL | TRIAE_CS42_3DL_TGACv1_250243_AA0864920.1 | 788 | 13 | 9 | 10 | PM | 559072825 | 559076602 |
TaHAK3-1BL | TRIAE_CS42_1BL_TGACv1_033126_AA0137190.1 | 818 | 11 | 6 | 7 | PM | 559097952 | 559092195 |
TaHAK3-1DL | TRIAE_CS42_1DL_TGACv1_062044_AA0207990.1 | 817 | 11 | 6 | 7 | PM | 414287464 | 414282613 |
TaHAK4-7AS | TRIAE_CS42_7AS_TGACv1_570480_AA1836390.3 | 686 | 11 | 4 | 5 | PM | 36648278 | 36651604 |
TaHAK4-7DS | TRIAE_CS42_7DS_TGACv1_622074_AA2032320.3 | 725 | 11 | 4 | 5 | PM | 36992201 | 36998396 |
TaHAK5-3AL | TRIAE_CS42_3AL_TGACv1_193587_AA0613950.2 | 788 | 11 | 9 | 10 | PM | 686834648 | 686837566 |
TaHAK5-3B | TRIAE_CS42_3B_TGACv1_222217_AA0760010.1 | 782 | 12 | 8 | 9 | PM | 729204607 | 729207978 |
TaHAK5-3DL | TRIAE_CS42_3DL_TGACv1_249200_AA0841080.1 | 764 | 11 | 9 | 10 | PM | 549623301 | 549628488 |
TaHAK6-3AL | TRIAE_CS42_3AL_TGACv1_195362_AA0648570.1 | 732 | 11 | 5 | 6 | PM | 689132246 | 689135499 |
TaHAK6-3D | TRIAE_CS42_U_TGACv1_646122_AA2146080.1 | 732 | 10 | 5 | 6 | PM | 552878679 | 552881787 |
TaHAK7-2AS | TRIAE_CS42_2AS_TGACv1_114941_AA0370050.1 | 778 | 13 | 8 | 9 | PM | 66798807 | 66805468 |
TaHAK7-2DS | TRIAE_CS42_2DS_TGACv1_179048_AA0603870.1 | 778 | 13 | 8 | 9 | PM | 67562036 | 67555857 |
TaHAK9-2AS | TRIAE_CS42_2AS_TGACv1_112740_AA0344400.1 | 782 | 11 | 6 | 7 | PM | 59558656 | 59554035 |
TaHAK9-2DS | TRIAE_CS42_2DS_TGACv1_177265_AA0571140.1 | 780 | 11 | 6 | 7 | PM | 58776915 | 58781534 |
TaHAK10-7AL | TRIAE_CS42_7AL_TGACv1_556774_AA1770580.1 | 829 | 11 | 8 | 9 | PM | 571701040 | 571706004 |
TaHAK10-7BL | TRIAE_CS42_7BL_TGACv1_578444_AA1895080.2 | 825 | 11 | 8 | 9 | PM | 532275080 | 532279899 |
TaHAK10-7DL | TRIAE_CS42_7DL_TGACv1_604392_AA1997720.1 | 827 | 11 | 8 | 9 | PM | 504174129 | 504179023 |
TaHAK11-2DL | TRIAE_CS42_2DL_TGACv1_159022_AA0530870.1 | 792 | 14 | 7 | 8 | PM | 533132266 | 533137640 |
TaHAK12-1AS | TRIAE_CS42_1AS_TGACv1_020203_AA0075600.3 | 790 | 14 | 8 | 9 | PM | 29186381 | 29192163 |
TaHAK13-7AL | TRIAE_CS42_7AL_TGACv1_557049_AA1775770.1 | 803 | 11 | 8 | 9 | PM | 665751743 | 665743564 |
TaHAK13-7BL | TRIAE_CS42_7BL_TGACv1_577019_AA1862890.1 | 750 | 11 | 9 | 10 | PM | 637233552 | 637228550 |
TaHAK13-7DL | TRIAE_CS42_7DL_TGACv1_603280_AA1979940.1 | 803 | 11 | 8 | 9 | PM | 575510919 | 575502903 |
TaHAK16-6AL | TRIAE_CS42_6AL_TGACv1_471561_AA1510890.1 | 806 | 11 | 9 | 10 | PM | 526114436 | 526120099 |
TaHAK16-6BL | TRIAE_CS42_6BL_TGACv1_502060_AA1622780.1 | 802 | 11 | 9 | 10 | PM | 574459052 | 574465508 |
TaHAK16-6DL | TRIAE_CS42_6DL_TGACv1_527466_AA1704460.1 | 805 | 11 | 9 | 10 | PM | 384217110 | 384223492 |
TaHAK17a-5AL | TRIAE_CS42_5AL_TGACv1_374155_AA1191940.2 | 719 | 12 | 7 | 8 | PM | 448614349 | 448619914 |
TaHAK17a-5BL | TRIAE_CS42_5BL_TGACv1_405525_AA1329450.1 | 719 | 12 | 7 | 8 | PM | 408955185 | 408959389 |
TaHAK17a-5DL | TRIAE_CS42_5DL_TGACv1_433406_AA1412260.1 | 719 | 12 | 7 | 8 | PM | 348479742 | 348483109 |
TaHAK17b-5DL | TRIAE_CS42_5DL_TGACv1_434380_AA1434940.1 | 712 | 11 | 7 | 8 | PM | 348346780 | 348352011 |
TaHAK18-5BL | TRIAE_CS42_5BL_TGACv1_407556_AA1357920.1 | 785 | 14 | 8 | 9 | PM | 546832103 | 546826324 |
TaHAK18-5DL | TRIAE_CS42_5DL_TGACv1_433145_AA1403590.1 | 785 | 14 | 8 | 9 | PM | 447886169 | 447880484 |
TaHAK19a-6AS | TRIAE_CS42_6AS_TGACv1_488672_AA1576040.1 | 735 | 11 | 5 | 6 | PM | 33582679 | 33586043 |
TaHAK19a-6BS | TRIAE_CS42_6BS_TGACv1_514904_AA1665660.1 | 737 | 11 | 5 | 6 | PM | 61222797 | 61226427 |
TaHAK19a-6D | TRIAE_CS42_U_TGACv1_644308_AA2138720.1 | 734 | 11 | 7 | 8 | PM | 28943744 | 28939705 |
TaHAK19b-6BS | TRIAE_CS42_6BS_TGACv1_513620_AA1645910.1 | 736 | 11 | 5 | 6 | PM | 60865245 | 60860866 |
TaHAK19b-6D | TRIAE_CS42_U_TGACv1_644372_AA2139050.1 | 738 | 11 | 6 | 7 | PM | 28998084 | 29001654 |
TaHAK19c-3B | TRIAE_CS42_3B_TGACv1_221313_AA0736890.1 | 745 | 10 | 6 | 7 | PM | 765005247 | 765001714 |
TaHAK19c-3DL | TRIAE_CS42_3DL_TGACv1_251389_AA0881060.1 | 744 | 10 | 6 | 7 | PM | 574916450 | 574919986 |
TaHAK22-2AL | TRIAE_CS42_2AL_TGACv1_096842_AA0321670.1 | 875 | 12 | 2 | 3 | PM | 392724188 | 392729472 |
TaHAK22-2BL | TRIAE_CS42_2BL_TGACv1_133206_AA0441840.1 | 873 | 12 | 2 | 3 | PM | 394364701 | 394359570 |
TaHAK22-2DL | TRIAE_CS42_2DL_TGACv1_158627_AA0523560.1 | 878 | 12 | 2 | 3 | PM | 325365084 | 325359910 |
TaHAK23-5AL | TRIAE_CS42_5AL_TGACv1_377379_AA1246300.1 | 814 | 10 | 9 | 10 | PM | 403355442 | 403350814 |
TaHAK23-5BL | TRIAE_CS42_5BL_TGACv1_406332_AA1344100.3 | 916 | 12 | 8 | 9 | PM | 355690579 | 355685671 |
TaHAK23-5DL | TRIAE_CS42_5DL_TGACv1_435669_AA1453280.1 | 898 | 12 | 8 | 9 | PM | 309789789 | 309784581 |
TaHAK24-7AS | TRIAE_CS42_7AS_TGACv1_569122_AA1807860.1 | 772 | 11 | 8 | 9 | PM | 172470095 | 172464552 |
TaHAK24-7BS | TRIAE_CS42_7BS_TGACv1_593324_AA1950450.2 | 771 | 13 | 8 | 9 | PM | 135583478 | 135578011 |
TaHAK24-7DS | TRIAE_CS42_7DS_TGACv1_621622_AA2021390.1 | 771 | 11 | 8 | 9 | PM | 169916315 | 169910689 |
TaHAK25-6AL | TRIAE_CS42_6AL_TGACv1_471048_AA1501690.1 | 769 | 12 | 8 | 9 | PM | 523950482 | 523944232 |
TaHAK25-6BL | TRIAE_CS42_6BL_TGACv1_501501_AA1618220.2 | 768 | 13 | 8 | 9 | PM | 570980883 | 570976570 |
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Cheng, X.; Liu, X.; Mao, W.; Zhang, X.; Chen, S.; Zhan, K.; Bi, H.; Xu, H. Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.). Int. J. Mol. Sci. 2018, 19, 3969. https://doi.org/10.3390/ijms19123969
Cheng X, Liu X, Mao W, Zhang X, Chen S, Zhan K, Bi H, Xu H. Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.). International Journal of Molecular Sciences. 2018; 19(12):3969. https://doi.org/10.3390/ijms19123969
Chicago/Turabian StyleCheng, Xiyong, Xiaodan Liu, Weiwei Mao, Xurui Zhang, Shulin Chen, Kehui Zhan, Huihui Bi, and Haixia Xu. 2018. "Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.)" International Journal of Molecular Sciences 19, no. 12: 3969. https://doi.org/10.3390/ijms19123969