Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of Silkworm TK Proteins
2.2. Chromosomal Distribution of the BmTK Gene Family
2.3. Comparative Analysis of TKs within Metazoans
2.4. Gene Ontology Analysis of the BmTKs
2.5. Spatial and Temporal Expression Profile of the BmTK Genes
3. Materials and Methods
3.1. Genome-Wide Identification of TK Proteins
3.2. Chromosomal Distribution and Gene Basic Information
3.3. Sequence Identity Matrix and Phylogenetic Analysis
3.4. Gene Ontology Annotation
3.5. Expression Profiling Based on DGE Sequencing and Microarray Data
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
TK | Tyrosine Kinase |
Bm | Bombyx mori |
kDa | kilo-Dalton |
RTK | Receptor TK |
CTK | Cytoplasmic TK (nonreceptor TK) |
References
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Type | Gene | Protein Length | Molecular Weight | Isoelectric Point | Chromosome |
---|---|---|---|---|---|
RTKs | BmDDR | 656 | 75,616.43 | 8.24 | chr1 |
BmEGFR | 1448 | 161,311.64 | 5.82 | chr1 | |
BmEPH1 | 1006 | 111,746 | 5.78 | chr1 | |
BmTRK | 578 | 64,646.3 | 5.43 | chr4 | |
BmRYK1 | 597 | 65,058.48 | 8.49 | chr5 | |
BmRYK2 | 542 | 60,476.73 | 6.98 | chr5 | |
BmFGFR1 | 856 | 97,292.72 | 5.86 | chr7 | |
BmFGFR2 | 616 | 69,437.85 | 5.61 | chr9 | |
BmROR | 971 | 109,667.49 | 8.05 | chr12 | |
BmVEGFR | 1366 | 155,204.04 | 5.85 | chr13 | |
BmAATYK | 3012 | 332,254.39 | 4.41 | chr17 | |
BmRET | 807 | 91,551.54 | 6.24 | chr19 | |
BmFGFR3 | 910 | 134,082.47 | 8.65 | chr22 | |
BmMUSK | 638 | 72,945.69 | 5.88 | chr22 | |
BmROS | 2464 | 276,014.61 | 6.56 | chr23 | |
BmALK | 1698 | 187,163.1 | 6.67 | chr24 | |
BmPTK7 | 184 | 21,040.15 | 6.31 | chr24 | |
BmMET | 739 | 83,289.74 | 5.83 | chr25 | |
BmEPH2 | 184 | 20,469.51 | 4.85 | unknown | |
CTKs | BmABL | 1247 | 136,157.76 | 8.31 | chr1 |
BmACK1 | 1136 | 127,807.11 | 6.84 | chr1 | |
BmINSR1 | 985 | 107,739.52 | 8.53 | chr4 | |
BmINSR2 | 1022 | 112,004.68 | 6.67 | chr4 | |
BmINSR3 | 1472 | 164,583.02 | 6.4 | chr5 | |
BmSYK | 803 | 90,867.3 | 6.34 | chr5 | |
BmFES | 867 | 98,133.68 | 8.38 | chr6 | |
BmACK2 | 911 | 102,923.58 | 6.89 | chr10 | |
BmSRC1 | 521 | 59,837.59 | 7.22 | chr11 | |
BmTEC | 657 | 74,694.1 | 8.49 | chr13 | |
BmJAK | 1074 | 123,869.62 | 6.75 | chr19 | |
BmSRC2 | 507 | 58,373.31 | 6.21 | chr19 | |
BmFAK | 1524 | 169,553.79 | 7.39 | chr20 | |
BmCSK | 589 | 64,243.5 | 8.31 | chr21 |
Type | Silkworm Gene Name | Protein Accession | Human Gene Name | Protein Accession | Identity |
---|---|---|---|---|---|
RTKs | BmAATYK | XP_021203756.1 | AATYK | NP_004911 | 29% |
BmALK | XP_012552890.1 | ALK | NP_004295 | 61% | |
BmDDR | NP_001108469.1 | DDR2 | NP_006173 | 42% | |
BmEGFR | XP_004929799.2 | ERBB4 | NP_005226 | 65% | |
BmEPH1 | XP_021207962.1 | EPHA5 | P54756 | 72% | |
BmEPH2 | Gene016321 | EPHA2 | NP_004422 | 47% | |
BmFGFR1 | NP_001037558.1 | FGFR3 | NP_000133 | 64% | |
BmFGFR2 | XP_004922880.2 | FGFR1 | AAA35835 | 48% | |
BmFGFR3 | BGIBMGA010869 | FGFR1 | AAA35835 | 37% | |
BmMET | XP_004925752.1 | RON | NP_002438 | 40% | |
BmMUSK | NP_001186538.1 | MUSK | NP_005583 | 62% | |
BmPTK7 | XP_021209072.1 | PTK7 | NP_002812 | 39% | |
BmRET | NP_001164049.1 | RET | P07949 | 44% | |
BmROR | XP_021203906.1 | ROR1 | NP_005003 | 55% | |
BmROS | XP_012551361.1 | ROS1 | NP_002935 | 59% | |
BmRYK1 | XP_021207735.1 | RYK | AAB26341 | 44% | |
BmRYK2 | XP_004932386.1 | RYK | AAB26341 | 50% | |
BmTRK | XP_004929369.2 | NTRK2 | NP_006171 | 46% | |
BmVEGFR | XP_012547297.1 | VEGFR1 | NP_002010 | 43% | |
CTKs | BmABL | XP_012551486.1 | ABL1 | NP_005148 | 77% |
BmACK1 | XP_004933204.2 | ACK1 | NP_005772 | 47% | |
BmACK2 | XP_004930430.1 | ACK1 | NP_005772 | 58% | |
BmCSK | XP_012548803.1 | CSK | NP_004374 | 57% | |
BmFAK | XP_021207025.1 | FAK | NP_005598 | 59% | |
BmFES | XP_012549644.1 | FES | NP_001996 | 57% | |
BmINSR1 | XP_021203435.1 | INSR | NP_000199 | 44% | |
BmINSR2 | XP_021203450.1 | INSRR | AAC31759 | 35% | |
BmINSR3 | NP_001037011.1 | IGF1R | NP_000866 | 71% | |
BmJAK | XP_021206252.1 | JAK2 | NP_004963 | 34% | |
BmSRC1 | XP_004932912.1 | FYN | NP_002028 | 57% | |
BmSRC2 | XP_012549151.1 | FYN | NP_002028 | 70% | |
BmSYK | XP_004930074.1 | ZAP70 | A44266 | 48% | |
BmTEC | XP_021204775.1 | TEC | NP_003206 | 58% |
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He, S.; Tong, X.; Han, M.; Bai, Y.; Dai, F. Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori. Int. J. Mol. Sci. 2018, 19, 934. https://doi.org/10.3390/ijms19040934
He S, Tong X, Han M, Bai Y, Dai F. Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori. International Journal of Molecular Sciences. 2018; 19(4):934. https://doi.org/10.3390/ijms19040934
Chicago/Turabian StyleHe, Songzhen, Xiaoling Tong, Minjin Han, Yanmin Bai, and Fangyin Dai. 2018. "Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori" International Journal of Molecular Sciences 19, no. 4: 934. https://doi.org/10.3390/ijms19040934