A Functional Mutation in KIAA1462 Promoter Decreases Glucocorticoid Receptor Affinity and Affects Egg-Laying Performance in Yangzhou Geese
Abstract
:1. Introduction
2. Results
2.1. SNP (Single Nucleotide Polymorphisms) Genotyping and Association Analysis
2.2. Sequence Characterization and Phylogenetic Relationships among Species of Yangzhou Geese KIAA1462 Gene
2.3. KIAA1462 mRNA Expression Profile in Yangzhou Geese Tissues
2.4. KIAA1462 mRNA Level Differs in Individuals with Different Genotypes
2.5. The mRNA Level of Caspase-3 Is Negative Regulated by KIAA1462
2.6. Direct Sequencing of the 5′ Flanking Region of KIAA1462
2.7. The c.-413C>G Mutation Causes Allele-Specific Binding of GR
2.8. GR mRNA Expression Profile in Yangzhou Geese Tissues
2.9. Glucocorticoid Promotes the Transcription Activity of the C But Not of the G Allele
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Animals and Samples Preparation
4.3. Genotyping and Association Analysis
4.4. RNA Isolation and First-Strand cDNA Synthesis
4.5. Cloning of KIAA1462 Gene Coding Sequence in Yangzhou Geese
4.6. Construction of pEGFP-KIAA1462-N1 Expression Vector
4.7. Quantitative Real-Time PCR Analysis
4.8. Granulosa Cell Culture and KIAA1462 Gene Overexpression
4.9. siRNA Preparation and Transfection
4.10. Mutation Detection in KIAA1462 Promoter Region
4.11. Electrophoretic Mobility Shift Assay (EMSA)
4.12. Dual Luciferase Reporter Assay
4.13. Bioinformatics Analysis
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
AMPK | Adenosine 5′-monophosphate (AMP)-activated protein kinase |
AS-PCR | Allele-Specific PCR |
CDS | Coding Sequence |
DMEM | Dulbecco’s modified Eagle’s medium |
EMSA | Electrophoretic Mobility Shift Assay |
GR | Glucocorticoid Receptor |
HFD | High-Fat Diet |
JCAD | Junctional Protein Associated with Coronary Artery Disease |
ORF | Open Reading Frame |
Q-PCR | Quantitative Real Time Polymerase Chain Reaction |
RAD sequencing | Restriction Site-Associated Sequencing |
SNP | Single-Nucleotide Polymorphism |
TOR | Target of Rapamycin |
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Genotype | Number of Geese | Number of Eggs | Lower Mean | Upper Mean | p Value |
---|---|---|---|---|---|
AA | 170 | 79.60 ± 11.08 a | 77.94 | 81.47 | 0.04 |
TA | 73 | 77.61 ± 11.11 a,b | 74.79 | 80.28 | |
TT | 13 | 73.00 ± 9.61 b | 69.62 | 76.23 | |
Total | 256 | 78.73 ± 11.61 | 77.43 | 80.11 |
Species | Nucleotide Accession Number | Amino Acid Accession Number | Nucleotide | Amino Acid |
---|---|---|---|---|
Platyrhynchos (Mallard) | XM_005027879.2 | XP_005027936 | 88.73% | 86.91% |
Gallus gallus (chicken) | XM_418578.5 | XP_418578.4 | 87.67% | 85.80% |
Meleagris gallopavo (turkey) | XM_003206999.2 | XP_003207047.2 | 86.68% | 84.01% |
Columba livia (Rock pigeon) | XM_005504208.1 | XP_005504265.1 | 83.74% | 80.19% |
Canis lupus familiaris (Dog) | XM_535151.5 | XP_535151.2 | 52.60% | 40.56% |
Homo sapiens (Human) | NM_020848.2 | NP_065899.1 | 55.01% | 40.68% |
Ovis aries (Sheep) | XM_004014272.3 | XP_004014321.1 | 51.04% | 35.64% |
Bos taurus (Cattle) | NM_001082474.1 | NP_001075943.1 | 50.68% | 36.66% |
Sus scrofa (Pig) | XM_003130706.5 | XP_003130754.3 | 52.06% | 36.67% |
Name | Sequences (5′→3′) | Function | Size (bp) | Tm (°C) |
---|---|---|---|---|
S1 | GCTGACAGCTCATTTGATA | AS-PCR | 67 | 58 |
S2 | GCTGACAGCTCATTTGATT | AS-PCR | ||
AS | CAGGATCACGTCCTCAAC | AS-PCR | ||
P1-F | ATGTTCAGTGTCGAGGACCTCC | Partial cDNA sequences | 1305 | 58 |
P1-R | AACAGAACGCAGGTAGTCA | |||
P2-F | GACCGCCTGCGAATAGTGT | Partial cDNA sequences | 1424 | 56 |
P2-R | CGTTTCCAACCTCCCACC | |||
P3-F | CTGAAGCCCGTAAGTCG | Partial cDNA sequences | 1300 | 56 |
P3-R | CTATTTGAGCGTCATTACGTGGG | |||
P4-F | CGAATTCCATGTTCAGTGTCGAGGACCTCC | KIAA1462 expression vector | 4029 | 62 |
P4-R | CCCCGGGGCTATTTGAGCGTCATTACGTGGG | |||
P5-F | AGCATGAGGTGCGTGGAGATG | q-PCR | 200 | 60 |
P5-R | CTCCAAACCCGAGTCTTGAACG | |||
caspase-3-F | CTGGTATTGAGGCAGACAGTGG | q-PCR | 158 | 50 |
caspase-3-R | CAGCACCCTACACAGAGACTGAA | |||
GAPDH-F | GCTGATGCTCCCATGTTCGTGAT | q-PCR | 86 | 60 |
GAPDH-R | GTGGTGCAAGAGGCATTGCTGAC | |||
GR-F | CTCTGGGTGTCATTACGGTGTT | q-PCR | 230 | 62 |
GR-R | CATTAGCTTGCTGGATTCCTTT | |||
KIAA1462-siRNA-1200 | GCCUGCGAAUAGUGUUGAATT | KIAA1462 knockdown experiments | ||
UUCAACACUAUUCGCAGGCTT | ||||
KIAA1462-siRNA-1847 | CCCAGAGCCCUGAUAAGAATT | KIAA1462 knockdown experiments | ||
UUCUUAUCAGGGCUCUGGGTT | ||||
KIAA1462-siRNA-2180 | CCAAACUGCUGUCUCCAAATT | KIAA1462 knockdown experiments | ||
UUUGGAGACAGCAGUUUGGTT | ||||
P6-F | TGGTTGATGTCCTGCGGG | Partial promoter sequences | 2440 | 59 |
P6-R | CCACCCTTACAATAAAGCACA | |||
P7-S1 | TCCCAGAATACAGAGCACTCC | AS-PCR | 265 | 58 |
P7-S2 | TCCCAGAATACAGAGCACTCG | AS-PCR | ||
P7-AS | CCACCCTTACAATAAAGCACATC | AS-PCR | ||
P8-F | GGGGTACCCCGGGAATCATTGAGACACGACA | Dual luciferase reporter vector | 630 | 64 |
P8-R | CCCTCGAGGGGCTGCTGAAGTGAAGGGTTT |
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Xia, M.; Wei, W.; Jiang, Z.; He, D.; Li, Z.; Yu, S.; Wang, Q.; Liu, H.; Chen, J. A Functional Mutation in KIAA1462 Promoter Decreases Glucocorticoid Receptor Affinity and Affects Egg-Laying Performance in Yangzhou Geese. Int. J. Mol. Sci. 2018, 19, 1531. https://doi.org/10.3390/ijms19051531
Xia M, Wei W, Jiang Z, He D, Li Z, Yu S, Wang Q, Liu H, Chen J. A Functional Mutation in KIAA1462 Promoter Decreases Glucocorticoid Receptor Affinity and Affects Egg-Laying Performance in Yangzhou Geese. International Journal of Molecular Sciences. 2018; 19(5):1531. https://doi.org/10.3390/ijms19051531
Chicago/Turabian StyleXia, Mengyuan, Wei Wei, Zaohang Jiang, Dandan He, Zhen Li, Shigang Yu, Qiushi Wang, Honglin Liu, and Jie Chen. 2018. "A Functional Mutation in KIAA1462 Promoter Decreases Glucocorticoid Receptor Affinity and Affects Egg-Laying Performance in Yangzhou Geese" International Journal of Molecular Sciences 19, no. 5: 1531. https://doi.org/10.3390/ijms19051531