Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model
Abstract
:1. Introduction
2. Results
2.1. The Effect of Periostracum Cicadae on Proteinuria and Other Biochemical Parameters
2.2. The Effect of Periostracum Cicadae on IgA
2.3. Periostracum Cicadae Alleviates Inflammation in Rats with IgAN
2.4. The Effect of Periostracum Cicadae on Inflammatory Cell Infiltration
2.5. Periostracum Cicadae Induces Cellular Apoptosis in the IgAN Rat Model
2.6. The Effect of Periostracum Cicadae on TGF-β1- and Fas-Positive Cells
2.7. Assessment of TLR4, MCP-1, IgA, and Caspase 3 Expression Levels
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Preparation of Prednisone Acetate Extract
4.3. Animals
4.4. Establishment of a IgAN Model
4.5. Sample Collection and Preparation
4.6. Biochemical Analysis of Serum Samples
4.7. Histopathological Examination
4.8. Immunofluorescence
4.9. TUNEL Assay
4.10. Immunohistochemical Staining
4.11. Western Blot Analysis
4.12. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Group | Controls | IgAN Model Group | Prednisone Acetate Group | Tripterygium Glycoside Tablet Group | Periostracum Cicadae Group | ||
---|---|---|---|---|---|---|---|
0.5 g/kg | 1 g/kg | 2 g/kg | |||||
IgA deposits | − | ++++ | + | ++ | +++ | ++ | + |
Control | BSA Was Replaced by Distilled Water; PBS Was Used as Injection Control | |
---|---|---|
IgAN | BSA, 4 mL/kg | Intragastrically every 2 days for 8 weeks |
CCL4/castor oil (25%, v/v) | subcutaneous injection (S.C.), 0.4 mL, once a week for 9 weeks | |
LPS, 0.05 mg | Caudal iv, once every two weeks for six weeks at the 6th week | |
25% ginger ale, 10 mL/kg | Once every two days for four consecutive weeks on the 9th week |
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Yang, L.; Wang, Y.; Nuerbiye, A.; Cheng, P.; Wang, J.-H.; Kasimu, R.; Li, H. Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model. Int. J. Mol. Sci. 2018, 19, 1599. https://doi.org/10.3390/ijms19061599
Yang L, Wang Y, Nuerbiye A, Cheng P, Wang J-H, Kasimu R, Li H. Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model. International Journal of Molecular Sciences. 2018; 19(6):1599. https://doi.org/10.3390/ijms19061599
Chicago/Turabian StyleYang, Lu, Yan Wang, Aobulikasimu Nuerbiye, Ping Cheng, Jin-Hui Wang, Rena Kasimu, and Hong Li. 2018. "Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model" International Journal of Molecular Sciences 19, no. 6: 1599. https://doi.org/10.3390/ijms19061599