Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows
Abstract
:1. Introduction
2. Results
2.1. Animal Performance Data
2.2. Effects of an n-3 Fatty Acid Reduced Diet and an Additional Fatty Acid Supplementation on the Fatty Acid Profile of the Plasma Membrane of RBCs
2.3. Flotillin-1 and Pannexin-1 Abundance in RBC Membranes
2.4. ATP Release from Bovine RBC
3. Discussion
4. Materials and Methods
4.1. Animals and Study Design
4.2. Blood Sampling and Plasma Membrane Extraction of RBC
4.3. Fatty Acid Analysis of Total Membrane Lipids
4.4. RBC Isolation and Measurement of ATP Release
4.5. Stimulated ATP Release from RBC in vitro, Incubated with LA and ALA
4.6. SDS-PAGE and Western Blot Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatment | P-Value | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Fatty Acid in RBC Membranes (µg/g) | ω | CTRL 1 | CLA 2 | EFA 3 | CLA + EFA 4 | SE 5 | Treatment | Dosage | Treatment × Dosage | Time 6 |
18:2, 9-cis,trans-11 | 0.042 | 0.289 | 0.248 | 0.000 | ||||||
Dosage III | 4.90 | 7.01 | 6.51 | 5.63 | 0.85 | |||||
Washout | 5.81 | 6.04 | 6.23 | 3.83 | 0.86 | |||||
18:2, trans-10,cis-12 | 0.250 | 0.157 | 0.290 | 0.000 | ||||||
Dosage III | 0.85 | 1.59 | 0.98 | 1.47 * | 0.27 | |||||
Washout | 0.92 | 1.24 | 0.99 | 0.57 | 0.27 | |||||
18:3 cis-9, cis-12, cis-15 | n3 | 0.161 | 0.008 | 0.000 | 0.000 | |||||
Dosage III | 8.32 b | 9.69 b | 17.25 *a | 16.56 *a | 1.34 | |||||
Washout | 12.51 * | 11.47 | 8.63 | 8.05 | 1.35 | |||||
20:3 cis-8,cis-11,cis-14 | n6 | 0.401 | 0.292 | 0.082 | 0.192 | |||||
Dosage III | 13.04 | 11.26 | 9.41 | 8.59 | 1.61 | |||||
Washout | 10.98 | 10.46 | 14.03 * | 11.13 | 1.63 | |||||
22:5 cis-7,cis-10,cis-13,cis-16,cis-19 | n3 | 0.503 | 0.227 | 0.108 | 0.575 | |||||
Dosage III | 6.55 | 9.81 | 12.47 | 12.40 * | 3.61 | |||||
Washout | 8.41 | 12.61 | 6.76 | 3.85 | 3.63 | |||||
24:1, 15c | 0.453 | 0.420 | 0.224 | 0.000 | ||||||
Dosage III | 0.25 | 0.49 | 0.21 | 0.55 * | 0.18 | |||||
Washout | 0.23 | 0.50 | 0.36 | −0.01 | 0.18 | |||||
Sum CLA | 0.082 | 0.220 | 0.228 | 0.000 | ||||||
Dosage III | 5.75 | 8.60 | 7.49 | 7.10 * | 1.05 | |||||
Washout | 6.73 | 7.28 | 7.21 | 4.40 | 1.06 | |||||
Sum n-3 | 0.583 | 0.058 | 0.004 | 0.108 | ||||||
Dosage III | 21.31 b | 27.18 ab | 37.42 *a | 35.90 *a | 4.66 | |||||
Washout | 28.76 | 31.04 | 21.96 | 18.19 | 4.69 | |||||
Sum n-6 | 0.696 | 0.937 | 0.777 | 0.751 | ||||||
Dosage III | 280.37 | 289.61 | 264.85 | 247.21 | 33.95 | |||||
Washout | 268.46 | 258.45 | 304.72 | 242.09 | 34.30 |
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Revskij, D.; Haubold, S.; Viergutz, T.; Kröger-Koch, C.; Tuchscherer, A.; Kienberger, H.; Rychlik, M.; Tröscher, A.; Hammon, H.M.; Schuberth, H.-J.; et al. Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows. Int. J. Mol. Sci. 2019, 20, 2769. https://doi.org/10.3390/ijms20112769
Revskij D, Haubold S, Viergutz T, Kröger-Koch C, Tuchscherer A, Kienberger H, Rychlik M, Tröscher A, Hammon HM, Schuberth H-J, et al. Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows. International Journal of Molecular Sciences. 2019; 20(11):2769. https://doi.org/10.3390/ijms20112769
Chicago/Turabian StyleRevskij, Denis, Susanne Haubold, Torsten Viergutz, Claudia Kröger-Koch, Armin Tuchscherer, Hermine Kienberger, Michael Rychlik, Arnulf Tröscher, Harald M. Hammon, Hans-Joachim Schuberth, and et al. 2019. "Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows" International Journal of Molecular Sciences 20, no. 11: 2769. https://doi.org/10.3390/ijms20112769