Sphingomyelin Synthase 2 Promotes Endothelial Dysfunction by Inducing Endoplasmic Reticulum Stress
Abstract
:1. Introduction
2. Results
2.1. SMS2 Can Activate ER Stress
2.2. SMS2 Can Trigger ER Stress by Provoking the Wnt/β-Catenin Pathway
2.3. Inhibition of ER Stress Can Decrease SMS2-Induced ED
2.4. Simvastatin Can Attenuate the ER Stress Induced by SMS2
2.5. Simvastatin Can Attenuate the Injury Induced by SMS2
2.6. Simvastatin Can Attenuate the Adhesion Capacity Induced by SMS2
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Transfection with an SMS2 Overexpression Plasmid
4.3. Measurement of the Degree of Oxidative Stress
4.4. Filipin Staining
4.5. Cell Adhesion Assay
4.6. Western Blot Analysis
4.7. LiCl or Salinomycin Treatment of HUVECs
4.8. Sphingomyelin Synthase Enzyme Activity Assay
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hua, L.; Wu, N.; Zhao, R.; He, X.; Liu, Q.; Li, X.; He, Z.; Yu, L.; Yan, N. Sphingomyelin Synthase 2 Promotes Endothelial Dysfunction by Inducing Endoplasmic Reticulum Stress. Int. J. Mol. Sci. 2019, 20, 2861. https://doi.org/10.3390/ijms20122861
Hua L, Wu N, Zhao R, He X, Liu Q, Li X, He Z, Yu L, Yan N. Sphingomyelin Synthase 2 Promotes Endothelial Dysfunction by Inducing Endoplasmic Reticulum Stress. International Journal of Molecular Sciences. 2019; 20(12):2861. https://doi.org/10.3390/ijms20122861
Chicago/Turabian StyleHua, Lingyue, Na Wu, Ruilin Zhao, Xuanhong He, Qian Liu, Xiatian Li, Zhiqiang He, Lehan Yu, and Nianlong Yan. 2019. "Sphingomyelin Synthase 2 Promotes Endothelial Dysfunction by Inducing Endoplasmic Reticulum Stress" International Journal of Molecular Sciences 20, no. 12: 2861. https://doi.org/10.3390/ijms20122861