Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor
Abstract
:1. Introduction
2. Results
3. Discussion
4. Methods
4.1. Animal Housing
4.2. Surgery
4.3. Experimental Protocol
- (1)
- Hypothermia (n = 5): After a 7-day recovery from surgery, animals were injected with the GABA-A agonist muscimol (1 mmoL) within the Raphe Pallidus (RPa). Each animal received, starting from 07:00 h, one injection/hour (100 nL). All animals entered synthetic torpor shortly after the first injection and maintained such condition until the end of the experiment.
- (2)
- Control (n = 5): After a 7-day recovery from surgery, animals were injected with artificial cerebrospinal fluid (ACSF) within the RPa. Each animal received, starting from 07:00 h, one injection/hour (100 nL).
4.4. Radiation Data
4.5. Gene Expression and Analysis
4.5.1. Liver
4.5.2. Testicle
4.5.3. Histology
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Tinganelli, W.; Hitrec, T.; Romani, F.; Simoniello, P.; Squarcio, F.; Stanzani, A.; Piscitiello, E.; Marchesano, V.; Luppi, M.; Sioli, M.; et al. Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor. Int. J. Mol. Sci. 2019, 20, 352. https://doi.org/10.3390/ijms20020352
Tinganelli W, Hitrec T, Romani F, Simoniello P, Squarcio F, Stanzani A, Piscitiello E, Marchesano V, Luppi M, Sioli M, et al. Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor. International Journal of Molecular Sciences. 2019; 20(2):352. https://doi.org/10.3390/ijms20020352
Chicago/Turabian StyleTinganelli, Walter, Timna Hitrec, Fabrizio Romani, Palma Simoniello, Fabio Squarcio, Agnese Stanzani, Emiliana Piscitiello, Valentina Marchesano, Marco Luppi, Maximiliano Sioli, and et al. 2019. "Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor" International Journal of Molecular Sciences 20, no. 2: 352. https://doi.org/10.3390/ijms20020352