Overexpression of ANAC046 Promotes Suberin Biosynthesis in Roots of Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. ANAC046 Localizes in the Nucleus and Acts as a Transcriptional Activator
2.2. Spatio-Temporal Expression of ANAC046 in Arabidopsis
2.3. Overexpression of ANAC046 Leads to an Increased Leaf, Silique and Seed Coat Permeability
2.4. ANAC046 Overexpression Leads to an Enhanced Expression of Suberin Biosynthesis Genes in Rosette Leaves
2.5. Overexpression of ANAC046 Promotes Deposition of Leaf Wax in Arabidopsis
2.6. Overexpression of ANAC046 Resulted in Increased Root Suberin Content
3. Discussion
4. Materials and Methods
4.1. Transcriptional Activity Assay of ANAC046 Using Yeast
4.2. Subcellular Localization of ANAC046
4.3. Generation of ANAC046 Transgenic Plants
4.4. GUS Staining
4.5. Toluidine Blue O Staining
4.6. Chlorophyll Leaching Assay
4.7. Quantitative RT-PCR Analysis of Genes Related Suberin and Cutin Biosynthesis Pathway
4.8. Analysis of Wax Composition in Leaves
4.9. Suberin Detection/Staining in the Roots Using Fluorol Yellow 088
4.10. Analysis of Suberin in Roots of Arabidopsis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
GUS | β-glucuronidase |
NAC | NAM (no apical meristem), ATAF1,2 and CUC2 (cup-shaped-cotyledon) |
SAGs | Senescence-Associated Genes |
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Mahmood, K.; Zeisler-Diehl, V.V.; Schreiber, L.; Bi, Y.-M.; Rothstein, S.J.; Ranathunge, K. Overexpression of ANAC046 Promotes Suberin Biosynthesis in Roots of Arabidopsis thaliana. Int. J. Mol. Sci. 2019, 20, 6117. https://doi.org/10.3390/ijms20246117
Mahmood K, Zeisler-Diehl VV, Schreiber L, Bi Y-M, Rothstein SJ, Ranathunge K. Overexpression of ANAC046 Promotes Suberin Biosynthesis in Roots of Arabidopsis thaliana. International Journal of Molecular Sciences. 2019; 20(24):6117. https://doi.org/10.3390/ijms20246117
Chicago/Turabian StyleMahmood, Kashif, Viktoria Valeska Zeisler-Diehl, Lukas Schreiber, Yong-Mei Bi, Steven J. Rothstein, and Kosala Ranathunge. 2019. "Overexpression of ANAC046 Promotes Suberin Biosynthesis in Roots of Arabidopsis thaliana" International Journal of Molecular Sciences 20, no. 24: 6117. https://doi.org/10.3390/ijms20246117