Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway
Abstract
:1. Introduction
2. Results
2.1. Plasma ALT and AST Activities
2.2. Hepatic NO Levels and iNOS Activity
2.3. Hepatic ROS Accumulation
2.4. Expressions of PI3K and AKT
2.5. Expressions of NF-κB
2.6. NF-κB Activation
2.7. Expressions of Inflammatory Mediators
2.8. Expressions of Anti-Inflammatory Mediators
3. Discussion
4. Materials and Methods
4.1. Protein Sources
4.2. Animals Experiments
4.3. Measurement of Plasma Enzyme Activity
4.4. Analyses of Hepatic NO Level and iNOS Activity
4.5. Determination of Hepatic ROS
4.6. Quantitative Real-Time PCR
4.7. Western Blotting Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Forward | Reverse |
---|---|---|
GAPDH | ACAGCAACAGGGTGGTGGAC | TTTGAGGGTGCAGCGAACTT |
NF-κB1 | TATGGGCAGGATGGACCTA | TCAGAGCCAAGAAAGGAAGC |
RelA | TGTGAACCAATTCGCCGAGAAGG | CTCAGCCAGCCAGTGCTTGTC |
IκBα | GAAGGACGAGGATTACGAGCAGATG | ATGGTCAGTGTCTTCTCTTCATGGATG |
Akt | ACTCATTCCAGACCCACGAC | AGCCCGAAGTCCGTTATCTT |
PI3K | TATTGCGAGGGAAACGAGAT | CCAGGGAGGTGTGTTGGTAA |
TNF-α | TGCCTCAGCCTCTTCTCATT | GCTTGGTGGTTTGCTACGAC |
IL-1β | TCACAGCAGCATCTCGACAA | GGTCCTCATCCTGGAAGCTC |
IL-6 | TCCGTTTCTACCTGGAGTTTG | GTTGGATGGTCTTGGTCCTT |
iNOS | GATGTGCTGCCTCTGGTCCT | GAGCTCCTGGAACCACTCGT |
COX-2 | AGCGACTGTTCCAAACCAGC | CCTCTTGGCGAGGGAGATGG |
MCP-1 | GGCCTGTTGTTCACAGTTGC | GTTCTCCAGCCGACTCATTG |
IL-10 | GCACTGCTATGTTGCCTGCT | TCAGCTCTCGGAGCATGTG |
HO-1 | GCCCTGGAAGAGGAGATAGAG | TAGTGCTGTGTGGCTGGTGT |
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Wang, Z.; Liang, M.; Li, H.; Cai, L.; Yang, L. Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway. Int. J. Mol. Sci. 2019, 20, 6164. https://doi.org/10.3390/ijms20246164
Wang Z, Liang M, Li H, Cai L, Yang L. Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway. International Journal of Molecular Sciences. 2019; 20(24):6164. https://doi.org/10.3390/ijms20246164
Chicago/Turabian StyleWang, Zhengxuan, Mingcai Liang, Hui Li, Liang Cai, and Lin Yang. 2019. "Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway" International Journal of Molecular Sciences 20, no. 24: 6164. https://doi.org/10.3390/ijms20246164