Different Signaling Pathways Define Different Interferon-Stimulated Gene Expression during Mycobacteria Infection in Macrophages
Abstract
:1. Introduction
2. Results
2.1. Mtb Infection Facilitates a Subset of ISG Production in Mϕs
2.2. ISG Production is Dependent on cGAS-STING and TLR-2/4 Signaling Pathways
2.3. Different ISGs Are Diversely Induced by TBK1, NF-κB, MAPK and JAK-STAT Signaling Pathways
2.4. A Number of ISGs in Mϕs of TB Patients are More Susceptible to Mycobacterial Infection or/and IFN-γ Treatment than that of Healthy People
3. Discussion
4. Materials and Methods
4.1. Study of Populations/Participants
4.2. Cell Culture of hMDMs and THP-1-Mϕs and Mtb Infection
4.3. Reagents
4.4. RNA Extraction and Reverse Transcription
4.5. Real Time PCR Quantitation of Cytokine mRNA
4.6. Protein Preparation and Western Blot Analysis
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhou, X.; Yang, J.; Zhang, Z.; Zhang, L.; Zhu, B.; Lie, L.; Huang, Y.; Ma, R.; Zhou, C.; Hu, S.; et al. Different Signaling Pathways Define Different Interferon-Stimulated Gene Expression during Mycobacteria Infection in Macrophages. Int. J. Mol. Sci. 2019, 20, 663. https://doi.org/10.3390/ijms20030663
Zhou X, Yang J, Zhang Z, Zhang L, Zhu B, Lie L, Huang Y, Ma R, Zhou C, Hu S, et al. Different Signaling Pathways Define Different Interferon-Stimulated Gene Expression during Mycobacteria Infection in Macrophages. International Journal of Molecular Sciences. 2019; 20(3):663. https://doi.org/10.3390/ijms20030663
Chicago/Turabian StyleZhou, Xinying, Jiahui Yang, Zelin Zhang, Lijie Zhang, Bo Zhu, Linmiao Lie, Yubin Huang, Rui Ma, Chaoying Zhou, Shengfeng Hu, and et al. 2019. "Different Signaling Pathways Define Different Interferon-Stimulated Gene Expression during Mycobacteria Infection in Macrophages" International Journal of Molecular Sciences 20, no. 3: 663. https://doi.org/10.3390/ijms20030663