BplMYB46 from Betula platyphylla Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses
Abstract
:1. Introduction
2. Results and Discussion
2.1. Sequence and Phylogenetic Analyses of Eight MYBs
2.2. Analysis of the Dimerization of BplMYB46
2.3. Verification by Transient Expression Assays
2.4. ChIP Analysis
2.5. Subcellular Localization of BplMYB46, 6, 8, 11, 12, and 13
2.6. Expression Patterns of BplMYB46, 6, 8, 11, 12, and 13 in Response to Abiotic Stresses
2.7. Plants Overexpressing BplMYB46 and BplMYB13 Display Alleviated Oxidative Stress and Diminished Cell Membrane Damage
2.8. Physiological Characterization of BplMYB46 and BplMYB13 Co-Overexpressing Plants
2.9. The Relative Expression of Target Genes
3. Materials and Methods
3.1. Plant Materials and Growth Conditions
3.2. Sequence Analysis of MYB Transcription Factors
3.3. Heterodimer and Homodimer Assays for BplMYB46
3.4. Transient Expression Assays
3.5. Chromatin Immunoprecipitation (ChIP) Analysis
3.6. Subcellular Localization Analysis
3.7. Plant Stress Treatments and Real-Time Reverse Transcription (RT)-PCR
3.8. Stress Tolerance Analysis of the Interaction of BplMYB46 with BplMYB13
3.9. Expression Analysis of Target Genes
3.10. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, Y.-M.; Wang, C.; Guo, H.-Y.; Wang, Y.-C. BplMYB46 from Betula platyphylla Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses. Int. J. Mol. Sci. 2019, 20, 1171. https://doi.org/10.3390/ijms20051171
Wang Y-M, Wang C, Guo H-Y, Wang Y-C. BplMYB46 from Betula platyphylla Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses. International Journal of Molecular Sciences. 2019; 20(5):1171. https://doi.org/10.3390/ijms20051171
Chicago/Turabian StyleWang, Yan-Min, Chao Wang, Hui-Yan Guo, and Yu-Cheng Wang. 2019. "BplMYB46 from Betula platyphylla Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses" International Journal of Molecular Sciences 20, no. 5: 1171. https://doi.org/10.3390/ijms20051171