Serial Femtosecond X-Ray Diffraction of HIV-1 Gag MA-IP6 Microcrystals at Ambient Temperature
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cloning and Overexpression of the HIV-1 Gag MA Domain
4.2. Crystallization of the HIV-1 Gag MA Domain
4.3. XFEL X-Ray Delivery and Detector
4.4. Injection of HIV-1 MA-IP6 Microcrystals into an XFEL and Diffraction Data Collection
4.5. Hit Finding
4.6. Indexing
5. Concluding Remarks
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Peak Finding Algorithm | Peak Criteria | Min. Peaks |
---|---|---|
Adaptive | Amax_thr 300, Atot_thr 600, Peak size 2–30, Son_min 10, Rank 3, Radius 3, Dr 2 | 15 |
Indexing Algorithm | Integration Radii | Unit Cell Tolerance |
---|---|---|
Mosflm, dirax | 3,4,5 | Axes lengths = 5% Axes angles = 1.5° |
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I Ciftci, H.; G Sierra, R.; Yoon, C.H.; Su, Z.; Tateishi, H.; Koga, R.; Kotaro, K.; Yumoto, F.; Senda, T.; Liang, M.; et al. Serial Femtosecond X-Ray Diffraction of HIV-1 Gag MA-IP6 Microcrystals at Ambient Temperature. Int. J. Mol. Sci. 2019, 20, 1675. https://doi.org/10.3390/ijms20071675
I Ciftci H, G Sierra R, Yoon CH, Su Z, Tateishi H, Koga R, Kotaro K, Yumoto F, Senda T, Liang M, et al. Serial Femtosecond X-Ray Diffraction of HIV-1 Gag MA-IP6 Microcrystals at Ambient Temperature. International Journal of Molecular Sciences. 2019; 20(7):1675. https://doi.org/10.3390/ijms20071675
Chicago/Turabian StyleI Ciftci, Halil, Raymond G Sierra, Chun Hong Yoon, Zhen Su, Hiroshi Tateishi, Ryoko Koga, Koiwai Kotaro, Fumiaki Yumoto, Toshiya Senda, Mengling Liang, and et al. 2019. "Serial Femtosecond X-Ray Diffraction of HIV-1 Gag MA-IP6 Microcrystals at Ambient Temperature" International Journal of Molecular Sciences 20, no. 7: 1675. https://doi.org/10.3390/ijms20071675