Roles of the Two-MnSOD System of Stenotrophomonas maltophilia in the Alleviation of Superoxide Stress
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analysis of Putative SODs in S. maltophilia
2.2. Implication of MnSODs and FeSOD in Aerobic Growth Conditions
2.3. Expression of the Three SODs in Response to Menadione and Metal Stresses
2.4. Implication of the Three SODs in Low-Iron and/or Superoxide Stress Conditions
2.5. Implication of the Three SODs in Low-Mn and/or Superoxide Stress Conditions
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Plasmid, and Growth Condition
4.2. Construction of Deletion Mutants KJΔA1, KJΔA2, and KJΔB
4.3. SOD Activity Assay
4.4. Construction of Promoter–xylE Transcriptional Fusion Plasmids, pSodA1xylE, pSodA2xylE, and pSodBxylE
4.5. Determination of C23O Activity
4.6. Quantitative Real-Time PCR (qRT–PCR)
4.7. Cell Viability Assay
4.8. Sequence and Phylogenetic Analyses
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Jair, H.-W.; Lu, H.-F.; Huang, Y.-W.; Pan, S.-Y.; Lin, I.-L.; Huang, H.-H.; Yang, T.-C. Roles of the Two-MnSOD System of Stenotrophomonas maltophilia in the Alleviation of Superoxide Stress. Int. J. Mol. Sci. 2019, 20, 1770. https://doi.org/10.3390/ijms20071770
Jair H-W, Lu H-F, Huang Y-W, Pan S-Y, Lin I-L, Huang H-H, Yang T-C. Roles of the Two-MnSOD System of Stenotrophomonas maltophilia in the Alleviation of Superoxide Stress. International Journal of Molecular Sciences. 2019; 20(7):1770. https://doi.org/10.3390/ijms20071770
Chicago/Turabian StyleJair, Herng-Woei, Hsu-Feng Lu, Yi-Wei Huang, Sz-Yun Pan, I-Ling Lin, Hsin-Hui Huang, and Tsuey-Ching Yang. 2019. "Roles of the Two-MnSOD System of Stenotrophomonas maltophilia in the Alleviation of Superoxide Stress" International Journal of Molecular Sciences 20, no. 7: 1770. https://doi.org/10.3390/ijms20071770