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Volume 21
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10.3390/ijms21124508
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Article

A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model

by
Ilia Banakh
1,2,
Perdita Cheshire
1,2,
Mostafizur Rahman
1,2,
Irena Carmichael
3,
Premlatha Jagadeesan
4,
Neil R. Cameron
4,
Heather Cleland
1,2 and
Shiva Akbarzadeh
1,2,*
1
Skin Bioengineering Laboratory, Victorian Adult Burns Service, Alfred Health, 89 Commercial Road, Melbourne VIC 3004, Australia
2
Department of Surgery, Monash University, 99 Commercial Road, Melbourne VIC 3004, Australia
3
Monash Micro Imaging, Monash University, 99 Commercial Road, Melbourne VIC 3004, Australia
4
Material Materials Science and Engineering, Monash University, 22 Alliance Lane, Clayton VIC 3800, Australia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(12), 4508; https://doi.org/10.3390/ijms21124508
Submission received: 5 June 2020 / Revised: 22 June 2020 / Accepted: 23 June 2020 / Published: 25 June 2020
(This article belongs to the Special Issue Molecular Mechanisms Related to Burns, Burn Wound Healing and Scarring)
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Abstract

Engineered dermal templates have revolutionised the repair and reconstruction of skin defects. Their interaction with the wound microenvironment and linked molecular mediators of wound repair is still not clear. This study investigated the wound bed and acellular “off the shelf” dermal template interaction in a mouse model. Full-thickness wounds in nude mice were grafted with allogenic skin, and either collagen-based or fully synthetic dermal templates. Changes in the wound bed showed significantly higher vascularisation and fibroblast infiltration in synthetic grafts when compared to collagen-based grafts (P ≤ 0.05). Greater tissue growth was associated with higher prostaglandin-endoperoxide synthase 2 (Ptgs2) RNA and cyclooxygenase-2 (COX-2) protein levels in fully synthetic grafts. Collagen-based grafts had higher levels of collagen III and matrix metallopeptidase 2. To compare the capacity to form a double layer skin substitute, both templates were seeded with human fibroblasts and keratinocytes (so-called human skin equivalent or HSE). Mice were grafted with HSEs to test permanent wound closure with no further treatment required. We found the synthetic dermal template to have a significantly greater capacity to support human epidermal cells. In conclusion, the synthetic template showed advantages over the collagen-based template in a short-term mouse model of wound repair.
Keywords: dermal templates; wound repair; human skin equivalent; graft; NovoSorb® BTM; Integra®; inflammation; COX-2 dermal templates; wound repair; human skin equivalent; graft; NovoSorb® BTM; Integra®; inflammation; COX-2

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MDPI and ACS Style

Banakh, I.; Cheshire, P.; Rahman, M.; Carmichael, I.; Jagadeesan, P.; Cameron, N.R.; Cleland, H.; Akbarzadeh, S. A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model. Int. J. Mol. Sci. 2020, 21, 4508. https://doi.org/10.3390/ijms21124508

AMA Style

Banakh I, Cheshire P, Rahman M, Carmichael I, Jagadeesan P, Cameron NR, Cleland H, Akbarzadeh S. A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model. International Journal of Molecular Sciences. 2020; 21(12):4508. https://doi.org/10.3390/ijms21124508

Chicago/Turabian Style

Banakh, Ilia, Perdita Cheshire, Mostafizur Rahman, Irena Carmichael, Premlatha Jagadeesan, Neil R. Cameron, Heather Cleland, and Shiva Akbarzadeh. 2020. "A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model" International Journal of Molecular Sciences 21, no. 12: 4508. https://doi.org/10.3390/ijms21124508

APA Style

Banakh, I., Cheshire, P., Rahman, M., Carmichael, I., Jagadeesan, P., Cameron, N. R., Cleland, H., & Akbarzadeh, S. (2020). A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model. International Journal of Molecular Sciences, 21(12), 4508. https://doi.org/10.3390/ijms21124508

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