Low-Dose Radiation Exposure with 56MnO2 Powder Changes Gene Expressions in the Testes and the Prostate in Rats
Abstract
:1. Introduction
2. Results
2.1. Estimated Doses of Internal Irradiation
2.2. Body and Testes Weight and Serum Testosterone Level
2.3. Histology of the Testes
2.4. Effects on mRNA Expression Levels of Leydig Cell-Specific Steroidogenesis Related Genes
2.5. Effects on mRNA Expression Levels of Sertoli Cell and Germ Cell-Specific Genes
2.6. Prostatic Secretory Protein mRNA Expressions
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Irradiation and Dosimetry
4.3. Measurement of mRNA Levels by Quantitative RT-PCR
4.4. Measurement of Serum Testosterone
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Groups | Body Weight (g) | Testes (g/kg bw) | Testosterone (pg/mL) | |
---|---|---|---|---|
Day 3 | Control | 248 ± 16 | 10.5 ± 1.1 | 1.2 ± 0.30 |
Cold Mn | 235 ± 14 | 11.4 ± 0.7 | 0.94 ± 0.13 | |
Mn56×1 | 235 ± 11 | 11.7 ± 0.6 | 0.7 ± 0.24 | |
Mn56×2 | 245 ± 16 | 11.3 ± 0.6 | 0.8 ± 0.17 | |
Mn56×3 | 237 ± 12 | 12.2 ± 0.6 | not determined | |
Co-60 | 234 ± 14 | 11.5 ± 0.8 | 1.15 ± 0.33 | |
Day 61 | Control | 330 ± 17 | 9.3 ± 0.7 | 1.45 ± 0.35 |
Cold Mn | 337 ± 19 | 9.8 ± 0.4 | 1.3 ± 0.14 | |
Mn56×1 | 371 ± 21 | 9.2 ± 0.6 | 1.42 ± 0.14 | |
Mn56×2 | 337 ± 17 | 9.1 ± 0.5 | 0.68 ± 0.26 * | |
Mn56×3 | 353 ± 17 | 9.1 ± 0.5 | 0.75 ± 0.23 | |
Co-60 | 328 ± 23 | 9.4 ± 0.5 | 0.59 ± 0.11 * |
Groups | PrstC3 (fmol/fmol βact) | CRP1 (fmol/fmol βact) | KS3 (fmol/fmol βact) | PSP94 (fmol/fmol βact) |
---|---|---|---|---|
Control | 651 ± 45 | 158 ± 22 | 20 ± 2.6 | 103 ± 7.3 |
Cold Mn | 566 ± 38 | 168 ± 26 | 13.5 ± 2.2 | 83 ± 11.2 |
Mn56 × 1 | 716 ± 82 | 206 ± 34 | 18.3 ± 2.9 | 86 ± 33.8 |
Mn56 × 2 | 611 ± 103 | 77 ± 14 * | 8.3 ± 1.6 ** | 75 ± 9.0 |
Mn56 × 3 | 453 ± 24 | 91 ± 10 | 9.8 ± 2.8 * | 49 ± 10.7 * |
Co-60 | 557 ± 79 | 79 ± 19 * | 11.8 ± 2.6 * | 71 ± 9.4 |
Gene | GenBank Accession# | Q-PCR Primer Sequences (5′–3′) | |
---|---|---|---|
Forward | Reverse | ||
Cyp11a1 | NM_017286 | TCCTCCCTGGTTACGTGCAG | GCAGAATAAGGAGCACCCCAG |
Cyp17a1 | NM_012753 | CAGCCAGATCAGTTCATGCCT | GACAAAGAGCTCCTGACGGG |
Hsd3b1 | NM_001007719 | AGAGAGATCTGGGCTATGTGCC | ACACCCAGAACCACATCCTTG |
StAR | NM_031558 | ACCTGCATGGTGCTTCATCC | GCTGGCGAACTCTATCTGGGT |
Cld11 | NM_053457 | TCCTCCCTGGTTACGTGCAG | GCAGAATAAGGAGCACCCCAG |
Clu | NM_053021 | TGCTTCATTCCCTCCAGTCC | TGGGTTGTCACTGTGGAGACC |
Spag4 | NM_031792 | CCAAGCTGATGATGACGAGACT | GGCCCCAGTTGCTTAAAATCT |
Zpbp | NM_001025139 | TTCAGCAAGTGGAAGTCCTGG | ACACAGCACTCAGGACACTTGG |
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Fujimoto, N.; Amantayeva, G.; Chaizhunussova, N.; Shabdarbayeva, D.; Abishev, Z.; Ruslanova, B.; Zhunussov, Y.; Azhimkhanov, A.; Zhumadilov, K.; Petukhov, A.; et al. Low-Dose Radiation Exposure with 56MnO2 Powder Changes Gene Expressions in the Testes and the Prostate in Rats. Int. J. Mol. Sci. 2020, 21, 4989. https://doi.org/10.3390/ijms21144989
Fujimoto N, Amantayeva G, Chaizhunussova N, Shabdarbayeva D, Abishev Z, Ruslanova B, Zhunussov Y, Azhimkhanov A, Zhumadilov K, Petukhov A, et al. Low-Dose Radiation Exposure with 56MnO2 Powder Changes Gene Expressions in the Testes and the Prostate in Rats. International Journal of Molecular Sciences. 2020; 21(14):4989. https://doi.org/10.3390/ijms21144989
Chicago/Turabian StyleFujimoto, Nariaki, Gaukhar Amantayeva, Nailya Chaizhunussova, Dariya Shabdarbayeva, Zhaslan Abishev, Bakhyt Ruslanova, Yersin Zhunussov, Almas Azhimkhanov, Kassym Zhumadilov, Aleksey Petukhov, and et al. 2020. "Low-Dose Radiation Exposure with 56MnO2 Powder Changes Gene Expressions in the Testes and the Prostate in Rats" International Journal of Molecular Sciences 21, no. 14: 4989. https://doi.org/10.3390/ijms21144989