Heterologous Expression of Poplar WRKY18/35 Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways
Abstract
:1. Introduction
2. Results
2.1. The Expression of PtrWRKY18 and PtrWRKY35 Is Induced by Various Hormones and Biotic Stresses
2.2. PtrWRKY18 and PtrWRKY35 Play Positive Roles in Resistance Against B. cinerea
2.3. PtrWRKY18 and PtrWRKY35 Play Negative Roles in Resistance Against P. syringae
2.4. Overexpression of PtrWRKY18 and PtrWRKY35 Reduced Sensitivity to ABA in Transgenic Arabidopsis
2.5. Overexpression of PtrWRKY18 and PtrWRKY35 Reduced Drought Tolerance in Arabidopsis
3. Discussion
3.1. SA, JA, and ABA All Play Important Roles in Regulating the Expression of PtrWRKY18 and PtrWRKY35
3.2. PtrWRKY18 and PtrWRKY35 Play Antagonistic Roles in JA and SA Signaling Pathway
3.3. PtrWRKY18 and PtrWRKY35 Play Roles in ABA Signal Perception, and Can Regulate the Tolerance to Osmotic Stress through Stomatal Movement
4. Materials and Methods
4.1. Plant Material and Treatments
4.2. GUS Staining
4.3. Chlorophyll Content Detection
4.4. Growth State Detection of P. syringae in Plants
4.5. Stomatal Movement Assay Response to ABA
4.6. Gene Expression Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Guo, L.; Li, C.; Jiang, Y.; Luo, K.; Xu, C. Heterologous Expression of Poplar WRKY18/35 Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways. Int. J. Mol. Sci. 2020, 21, 5440. https://doi.org/10.3390/ijms21155440
Guo L, Li C, Jiang Y, Luo K, Xu C. Heterologous Expression of Poplar WRKY18/35 Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways. International Journal of Molecular Sciences. 2020; 21(15):5440. https://doi.org/10.3390/ijms21155440
Chicago/Turabian StyleGuo, Li, Chaofeng Li, Yuanzhong Jiang, Keming Luo, and Changzheng Xu. 2020. "Heterologous Expression of Poplar WRKY18/35 Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways" International Journal of Molecular Sciences 21, no. 15: 5440. https://doi.org/10.3390/ijms21155440