Novel Insights into the Regulatory Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 in Oxidative Stress and Inflammation of Human Fetal Membranes
Abstract
:1. Introduction
2. Results
2.1. CSE and LPS Increase Nrf2 Expressions in Human Fetal Membranes
2.2. SFN Co-Treatment Augments CSE and LPS Response and Increase Nrf2
2.3. SFN Co-Treatment with CSE or LPS Increases PPARγ Expression
2.4. TRN Co-Treatment with CSE and LPS Did Not Change Nrf2 and PPARγ Expressions
2.5. Endogenous HO-1 Levels are Increased by SFN Co-Treatment of CSE and LPS
2.6. SFN Reduces IL-6 Release Induced by Both CSE and LPS
3. Discussion
4. Materials and Methods
4.1. IRB Ethics Committee Approval Statement
4.2. Tissue-Culture of Normal Term Fetal Membranes
4.3. Water-Soluble Cigarette Smoke Extract (CSE) Preparation and Stimulation of the Fetal Membranes with CSE and LPS
4.4. Treatment with SFN and TRN
4.5. Western Blot Analysis for Nrf2 and PPARγ
4.6. HO-1 ELISA
4.7. Luminex Assay to Determine Cytokine Concentration in Culture Supernatants
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Menon, R.; Peltier, M.R. Novel Insights into the Regulatory Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 in Oxidative Stress and Inflammation of Human Fetal Membranes. Int. J. Mol. Sci. 2020, 21, 6139. https://doi.org/10.3390/ijms21176139
Menon R, Peltier MR. Novel Insights into the Regulatory Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 in Oxidative Stress and Inflammation of Human Fetal Membranes. International Journal of Molecular Sciences. 2020; 21(17):6139. https://doi.org/10.3390/ijms21176139
Chicago/Turabian StyleMenon, Ramkumar, and Morgan R Peltier. 2020. "Novel Insights into the Regulatory Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 in Oxidative Stress and Inflammation of Human Fetal Membranes" International Journal of Molecular Sciences 21, no. 17: 6139. https://doi.org/10.3390/ijms21176139