The Development of Hyaluronan/Fucoidan-Based Nanoparticles as Macrophages Targeting an Epigallocatechin-3-Gallate Delivery System
Abstract
:1. Introduction
2. Results
2.1. Effects of Fucoidan on Cell Migration Ability
2.2. Effects of Hyaluronan on CD44-Targeting Ability
2.3. Synthesis and Characterization of the EGCG-Loaded Hyaluronan/Fucoidan/PEG–Gelatin Nanoparticles
2.4. Macrophage Cellular Internalization of Nanoparticles and Uptake of EGCG
2.5. Anti-Inflammatory Effects of EGCG-Loaded Nanoparticles
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Assay of the Effects of Fucoidan on Macrophage Cell Migration
4.3. Assay of Hyaluronan Binding to CD44 Protein and Macrophage Cells
4.4. Preparation and Characterization of Nanoparticles
4.5. Cellular Internalization of Nanoparticles and the Uptake of EGCG
4.6. Western Blotting Analysis of Inflammatory-Related Proteins
4.7. Assay of Inflammatory Cytokine Interleukin-6 (IL-6) and Nitric Oxide (NO) Levels
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BSA | bovine serum albumin |
Cy5 | cyanine 5 hydrazide |
DAPI | 4′,6-diamidino-2-phenylindole |
EGCG | (−)-epigallocatechin 3-gallate |
FLA | fluoresceinamine |
FBS | fetal bovine serum |
FTIR | Fourier transform infrared spectroscopy |
HPLC | high-performance liquid chromatography |
IL-6 | interleukin-6 |
LPS | lipopolysaccharides |
NO | nitric oxide |
NF-κB | nuclear factor-kappa B |
PEG | polyethylene glycol |
PBS | phosphate-buffered saline |
R6G | rhodamine 6G |
SD | standard deviation |
TEM | transmission electron microscope |
VT750 | vivotag 750-NHS ester |
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PEG–Gelatin 3.750 mg/mL; EGCG 2.000 mg/mL | ||||
---|---|---|---|---|
Hyaluronan/Fucoidan (mg/mL) | Mean Particle Size (nm) | Polydispersity Indices | Zeta Potential Value (mV) | Egcg Loading Efficiency (%) |
0.625:0.625 | 244.00 ± 12.00 | 0.30 ± 0.05 | −29.86 ± 1.56 | 39.86 ± 4.88 |
1.250:1.250 | 217.00 ± 14.00 | 0.28 ± 0.07 | −33.60 ± 1.30 | 52.08 ± 5.37 |
1.875:1.875 | 279.00 ± 16.00 | 0.38 ± 0.02 | −37.76 ± 0.67 | 62.66 ± 3.21 |
2.500:2.500 | 454.00 ± 28.00 | 0.45 ± 0.10 | −37.72 ± 1.16 | 70.12 ± 2.09 |
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Ho, C.-H.; Chu, P.-Y.; Peng, S.-L.; Huang, S.-C.; Lin, Y.-H. The Development of Hyaluronan/Fucoidan-Based Nanoparticles as Macrophages Targeting an Epigallocatechin-3-Gallate Delivery System. Int. J. Mol. Sci. 2020, 21, 6327. https://doi.org/10.3390/ijms21176327
Ho C-H, Chu P-Y, Peng S-L, Huang S-C, Lin Y-H. The Development of Hyaluronan/Fucoidan-Based Nanoparticles as Macrophages Targeting an Epigallocatechin-3-Gallate Delivery System. International Journal of Molecular Sciences. 2020; 21(17):6327. https://doi.org/10.3390/ijms21176327
Chicago/Turabian StyleHo, Chang-Hsun, Pei-Yi Chu, Shin-Lei Peng, Shun-Chih Huang, and Yu-Hsin Lin. 2020. "The Development of Hyaluronan/Fucoidan-Based Nanoparticles as Macrophages Targeting an Epigallocatechin-3-Gallate Delivery System" International Journal of Molecular Sciences 21, no. 17: 6327. https://doi.org/10.3390/ijms21176327