Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins
Abstract
:1. Introduction
2. Results
2.1. Sequencing and Read Mapping
2.2. Transcript Differential Expression in NB and IB Fed Mosquitoes (In Vivo Experiments)
2.3. Transcript Differential Expression in Aag2 Cells upon Treatment with hC3 and hC5a (In Vitro Experiments)
2.4. Changes in the Immune Response Transcripts (IRTs) to Complement in the In Vivo and In Vitro Experiments
2.5. Shared Transcripts between the In Vivo and the In Vitro Assays
3. Discussion
4. Materials and Methods
4.1. Blood Source
4.2. Maintenance of Ae. aegypti Adult Mosquitoes and Cell Line
4.3. Mosquito Blood-Feeding
4.4. Aag2 Cells Exposure to Human Complement Protein hC3 and hC5a
4.5. Mosquito RNA Isolation and Sequencing
4.6. RNA-Seq Differential Expression Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
YFV | Yellow fever virus |
DENV | Dengue virus |
CHIKV | Chikungunya virus |
ZIKV | Zika virus |
MAC | Membrane attack complex |
hC3 | Human complement protein C3 |
hC5a | Human complement protein C5a |
AaMCR | Ae. aegypti macroglobulin complement-related proteins |
AMP | Antimicrobial peptides |
TBV | Transmission blocking vaccines |
LVP_AGWG | Liverpool Aedes Genome Working Group |
STAR | Spliced Transcripts Alignment to a Reference |
RSEM | RNA-Seq by Expectation-Maximization |
FDR | False discovery rate |
CPM | Counts per Million |
TMM | Trimmed Mean of M-values |
DE | Differentially expressed |
IB | Blood with heat-inactivated plasma |
NB | Blood with normal plasma |
SF | Sugar-fed |
FC | Fold change |
GSEA | Gene Set Enrichment Analysis |
MF | Molecular functions |
GO | Gene Ontology |
IRT | Immune response transcripts |
5G1 | Late-phase trypsin |
ACA | Anticomplementary activity |
C5aR1 | C5a receptor 1 (CD88) |
C5aR2 | C5a receptor 2 (C5L2) |
GTP | Guanosine-5’-triphosphate |
EDTA | Ethylenediaminetetraacetic acid |
RBC | Red blood cells |
PBS | Phosphate Buffered Saline |
BME | Basal Medium Eagle |
KOAPF | K-State Open Access Publishing Fund |
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Transcript_ID | Gene Description (Gene Ontology Molecular Function and Description) | Log2FC | ||
---|---|---|---|---|
hC3/Ctl | hC5a/Ctl | NB/IB | ||
AAEL001698-RB | Charged multivesicular body protein 4b (GO:0007034, vacuolar transport) | 2.80 | 2.06 | 5.46 |
AAEL007290-RE | None (None) | 2.93 | 2.90 | 3.06 |
AAEL001447-RG | None | 3.40 | 4.26 | 3.91 |
AAEL002557-RF | Cationic amino acid transporter (GO:0022857, transmembrane transporter activity) | 3.41 | 2.91 | 5.27 |
AAEL005460-RD | alpha-galactosidase/alpha-n-acetylgalactosaminidase (GO:0003824;GO:0016787;GO:0016798;GO:0004553, catalytic activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds) | 3.78 | 3.75 | 3.54 |
AAEL025517-RB | None (GO:0005524;GO:0004672, ATP binding; protein kinase activity) | 3.79 | 3.47 | 5.92 |
AAEL012899-RB | Meiotic coiled-coil protein, putative GO:0003690, double-stranded DNA binding) | 3.96 | 3.58 | 6.30 |
AAEL026355-RA | None (GO:0005515, protein binding) | 4.25 | 3.23 | 4.85 |
AAEL008574-RB | Acyl-CoA oxidase (GO:0071949;GO:0003997;GO:0050660;GO:0016627, FAD binding; acyl-CoA oxidase activity; flavin adenine dinucleotide binding) | 4.45 | 4.36 | 5.09 |
AAEL009249-RC | Coronin (GO:0005515, protein binding) | 4.58 | 5.13 | 4.97 |
AAEL009212-RP | Lola (GO:0005515, protein binding) | 4.86 | 4.88 | 6.08 |
AAEL007845-RF | Rab5 (GO:0005525;GO:0003924, GTP binding; GTPase activity) | 4.88 | 5.16 | 6.89 |
AAEL001557-RC | Mucolipin (GO:0005261, cation channel activity) | 4.89 | 2.70 | 5.76 |
AAEL005022-RB | Mediator of RNA polymerase II transcription subunit 15 (Med15) (GO:0003712, transcription coregulator activity) | 5.01 | 5.26 | 8.12 |
AAEL003762-RD | Protoporphyrinogen IX oxidase (GO:0016491;GO:0004729, oxidoreductase activity; oxygen-dependent protoporphyrinogen oxidase activity) | 5.15 | 6.23 | 3.13 |
AAEL015598-RA | None (GO:0016787, hydrolase activity) | 5.21 | 2.97 | 2.24 |
AAEL019780-RB | None (GO:0008410, CoA-transferase activity) | 5.27 | 5.19 | 2.62 |
AAEL005023-RD | Palmitoyltransferase (None) | 5.33 | 6.30 | 5.14 |
AAEL021339-RF | None (None) | 5.50 | 3.79 | 3.93 |
AAEL004931-RA | Beta-hexosaminidase b (GO:0102148;GO:0004563;GO:0016787;GO:0016798;GO:0004553, N-acetyl-beta-D-galactosaminidase activity; beta-N-acetylhexosaminidase activity) | 5.67 | 3.41 | 6.73 |
AAEL024186-RC | None (GO:0003779;GO:0030276;GO:0005543, actin binding; clathrin binding; phospholipid binding) | 5.87 | 3.00 | 6.26 |
AAEL006633-RB | Inhibitor of Apoptosis (IAP) (None) | −7.22 | −7.08 | −4.47 |
AAEL006510-RC | None (GO:0003723;GO:0008190, RNA binding; eukaryotic initiation factor 4E binding) | −6.61 | −6.04 | −6.91 |
AAEL012000-RA | None (None) | −6.42 | −6.29 | −4.70 |
AAEL009059-RB | Arp2/3 complex 16 kd subunit (P16-arc) (None) | −5.86 | −5.74 | −5.88 |
AAEL001041-RN | Guanine nucleotide-binding protein beta 3 (g protein beta3) (GO:0005515, protein binding) | −5.72 | −5.00 | −4.14 |
AAEL000234-RN | Class B Scavenger Receptor (CD36 domain) (None) | −5.50 | −3.75 | −3.05 |
AAEL012410-RB | Eukaryotic translation initiation factor 2C (GO:0003676;GO:0005515, nucleic acid binding; protein binding) | −5.50 | −4.20 | −3.32 |
AAEL008542-RC | Kinesin heavy chain subunit (GO:0005524;GO:0008017;GO:0003777, ATP binding; microtubule binding; microtubule motor activity) | −5.04 | −4.91 | −4.09 |
AAEL023848-RA | None (None) | −4.87 | −2.81 | −6.23 |
AAEL007874-RC | None (GO:0047372, acylglycerol lipase activity) | −4.76 | −4.63 | −4.37 |
AAEL004496-RD | Glutamate transporter (GO:0015293, symporter activity) | −4.66 | −4.52 | −5.88 |
AAEL010373-RK | Dullard protein (GO:0016791, phosphatase activity) | −4.29 | −4.16 | −4.68 |
AAEL006364-RC | None (GO:0045127, N-acetylglucosamine kinase activity) | −4.24 | −4.11 | −7.08 |
AAEL004420-RC | None (None) | −4.19 | −4.06 | −5.29 |
AAEL011858-RD | Transcription factor IIIA (GO:0003676;GO:0008270, nucleic acid binding; zinc ion binding) | −4.19 | −4.07 | −3.54 |
AAEL006982-RG | Lipase (GO:0052689, carboxylic ester hydrolase activity) | −4.18 | −4.07 | −2.50 |
AAEL025570-RE | None (GO:0005524;GO:0003774;GO:0005515, ATP binding; motor activity; protein binding) | −4.12 | −6.57 | −2.59 |
AAEL004385-RB | UGA suppressor tRNA-associated antigenic protein (GO:0003824;GO:0016740;GO:0016785, catalytic activity; transferase activity; transferase activity) | −4.11 | −3.98 | −5.09 |
AAEL018032-RE | None (None) | −3.97 | −3.43 | −6.07 |
AAEL000549-RE | Fibulin 1 (GO:0005509;GO:0005515, calcium ion binding; protein binding) | −3.75 | −3.62 | −4.69 |
AAEL000354-RB | Dimeric dihydrodiol dehydrogenase (GO:0016491, oxidoreductase activity) | −3.69 | −3.56 | −3.09 |
AAEL006556-RA | None (None) | −2.47 | −2.43 | −5.21 |
AAEL003471-RA | None (GO:0003676;GO:0008270, nucleic acid binding; zinc ion binding) | −2.44 | −2.40 | −3.73 |
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Giraldo-Calderón, G.I.; Calle-Tobón, A.; Rozo-López, P.; Colpitts, T.M.; Park, Y.; Rua-Uribe, G.L.; Londono-Renteria, B. Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins. Int. J. Mol. Sci. 2020, 21, 6584. https://doi.org/10.3390/ijms21186584
Giraldo-Calderón GI, Calle-Tobón A, Rozo-López P, Colpitts TM, Park Y, Rua-Uribe GL, Londono-Renteria B. Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins. International Journal of Molecular Sciences. 2020; 21(18):6584. https://doi.org/10.3390/ijms21186584
Chicago/Turabian StyleGiraldo-Calderón, Gloria I., Arley Calle-Tobón, Paula Rozo-López, Tonya M. Colpitts, Yoonseong Park, Guillermo L. Rua-Uribe, and Berlin Londono-Renteria. 2020. "Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins" International Journal of Molecular Sciences 21, no. 18: 6584. https://doi.org/10.3390/ijms21186584