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Article

Transcriptome Analysis Reveals the Neuro-Immune Interactions in Duck Tembusu Virus-Infected Brain

by
Junqin Zhang
,
Yunzhen Huang
,
Linlin Li
,
Jiawen Dong
,
Ming Liao
* and
Minhua Sun
*
Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture, Guangdong Open Laboratory of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2402; https://doi.org/10.3390/ijms21072402
Submission received: 12 March 2020 / Revised: 28 March 2020 / Accepted: 28 March 2020 / Published: 31 March 2020
(This article belongs to the Special Issue Molecular Research on Vector-Borne Diseases of Medical Interest: From Bench to Application)
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Abstract

The duck Tembusu virus (DTMUV) is a mosquito-borne flavivirus. It causes severe symptoms of egg-drop, as well as neurological symptoms and brain damage in ducks. However, the specific molecular mechanisms of DTMUV-induced neurovirulence and host responses in the brain remain obscure. To better understand the host–pathogen and neuro-immune interactions of DTMUV infection, we conducted high-throughput RNA-sequencing to reveal the transcriptome profiles of DTMUV-infected duck brain. Totals of 117, 212, and 150 differentially expressed genes (DEGs) were identified at 12, 24, and 48 h post infection (hpi). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses uncovered genes and pathways related to the nervous system and immune responses in duck brain. Neuro-related genes, including WNT3A, GATA3, and CHRNA6, were found to be significantly downregulated. RIG-I-like receptors (DHX58, IFIH1) and Toll-like receptors (TLR2 and TLR3) were activated, inducing the expression of 22 interferon stimulated genes (ISGs) and antigen-processing and -presenting genes (TAP1 and TAP2) in the brain. Our research provides comprehensive information for the molecular mechanisms of neuro-immune and host–pathogen interactions of DTMUV.
Keywords: Transcriptome analysis; DTMUV; brain; molecular mechanism; neuro-immune interaction; host–pathogen interaction Transcriptome analysis; DTMUV; brain; molecular mechanism; neuro-immune interaction; host–pathogen interaction

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MDPI and ACS Style

Zhang, J.; Huang, Y.; Li, L.; Dong, J.; Liao, M.; Sun, M. Transcriptome Analysis Reveals the Neuro-Immune Interactions in Duck Tembusu Virus-Infected Brain. Int. J. Mol. Sci. 2020, 21, 2402. https://doi.org/10.3390/ijms21072402

AMA Style

Zhang J, Huang Y, Li L, Dong J, Liao M, Sun M. Transcriptome Analysis Reveals the Neuro-Immune Interactions in Duck Tembusu Virus-Infected Brain. International Journal of Molecular Sciences. 2020; 21(7):2402. https://doi.org/10.3390/ijms21072402

Chicago/Turabian Style

Zhang, Junqin, Yunzhen Huang, Linlin Li, Jiawen Dong, Ming Liao, and Minhua Sun. 2020. "Transcriptome Analysis Reveals the Neuro-Immune Interactions in Duck Tembusu Virus-Infected Brain" International Journal of Molecular Sciences 21, no. 7: 2402. https://doi.org/10.3390/ijms21072402

APA Style

Zhang, J., Huang, Y., Li, L., Dong, J., Liao, M., & Sun, M. (2020). Transcriptome Analysis Reveals the Neuro-Immune Interactions in Duck Tembusu Virus-Infected Brain. International Journal of Molecular Sciences, 21(7), 2402. https://doi.org/10.3390/ijms21072402

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