Functional Characterization of a Putative RNA Demethylase ALKBH6 in Arabidopsis Growth and Abiotic Stress Responses
Abstract
:1. Introduction
2. Results
2.1. The Domain Structure, Subcellular Localization, and Stress-Responsive Expression Pattern of ALKBH6 in Arabidopsis
2.2. ALKBH6 Affects Seed Germination and Seedling Growth under Abiotic Stresses
2.3. ALKBH6 Influences the Response of Arabidopsis to ABA
2.4. ALKBH6 Binds to the RNA with m6A or m5C Modification
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Germination and Seedling Growth Assays under Abiotic Stresses
4.3. Analysis of Subcellular Localization of the ALKBH6 Protein
4.4. RNA Extraction and Real-Time RT-PCR
4.5. Vector Construction and the Expression of Recombinant Protein in Escherichia coli
4.6. RNA-Protein Binding Assay
4.7. Measurement of the m6A/A and m5C levels
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ALKBH | AlkB homolog |
ABA | Abscisic acid |
GFP | Green fluorescent protein |
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Huong, T.T.; Ngoc, L.N.T.; Kang, H. Functional Characterization of a Putative RNA Demethylase ALKBH6 in Arabidopsis Growth and Abiotic Stress Responses. Int. J. Mol. Sci. 2020, 21, 6707. https://doi.org/10.3390/ijms21186707
Huong TT, Ngoc LNT, Kang H. Functional Characterization of a Putative RNA Demethylase ALKBH6 in Arabidopsis Growth and Abiotic Stress Responses. International Journal of Molecular Sciences. 2020; 21(18):6707. https://doi.org/10.3390/ijms21186707
Chicago/Turabian StyleHuong, Trinh Thi, Le Nguyen Tieu Ngoc, and Hunseung Kang. 2020. "Functional Characterization of a Putative RNA Demethylase ALKBH6 in Arabidopsis Growth and Abiotic Stress Responses" International Journal of Molecular Sciences 21, no. 18: 6707. https://doi.org/10.3390/ijms21186707