Selective Elimination of NG2-Expressing Hair Follicle Stem Cells Exacerbates the Sensitization Phase of Contact Dermatitis in a Transgenic Rat Model
Abstract
:1. Introduction
2. Results
2.1. Expression of HSVtk in NG2+ HFSCs of Tg Rats
2.2. GCV Induced Programmed Cell Death in the Dividing NG2+ Cells of the Hair Follicles during Anagen
2.3. Selective Elimination of NG2+ HFSCs during Anagen-Induced Contact Dermatitis with a Depilatory Cream
2.4. Selective Ablation of NG2+ HFSCs during Anagen Exacerbated the Sensitization Phase of DNFB-Induced Contact Dermatitis
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Selective Elimination of NG2+ HFSCs during Depilation-Induced Anagen
4.3. DNFB-Induced Contact Dermatitis
4.4. Immunohistochemistry
4.5. Real-Time Polymerase Chain Reaction Assay
4.6. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
Abbreviations
HFSCs | hair follicle stem cells |
HSVtk | herpes simplex virus thymidine kinase |
GCV | ganciclovir |
Tg | transgenic |
sHG | secondary hair germ |
CSPG4 | chondroitin sulfate proteoglycan 4 |
DNFB | dinitrofluorobenzene |
TUNEL | terminal deoxynucleotidyl transferase dUDP nick-end labeling |
WT | Wild type |
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Depilatory Cream | Redness (Erythema) | More Severe (Dry, Cracked, Scaly, Skin) | |
---|---|---|---|
GCV group | + | 15/15 | 13/15 |
Vehi group | + | 0/10 | 0/10 |
GCV group | - | 1/8 | 0/8 |
Vehi group | - | 0/5 | 0/5 |
DNFB | Redness (Erythema) | More Severe (Dry, Cracked, Scaly, Skin) | |
---|---|---|---|
GCV group | + | 4/4 | 4/4 |
Vehi group | + | 4/4 | 0/4 |
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Tamura, Y.; Takata, K.; Eguchi, A.; Kataoka, Y. Selective Elimination of NG2-Expressing Hair Follicle Stem Cells Exacerbates the Sensitization Phase of Contact Dermatitis in a Transgenic Rat Model. Int. J. Mol. Sci. 2020, 21, 6922. https://doi.org/10.3390/ijms21186922
Tamura Y, Takata K, Eguchi A, Kataoka Y. Selective Elimination of NG2-Expressing Hair Follicle Stem Cells Exacerbates the Sensitization Phase of Contact Dermatitis in a Transgenic Rat Model. International Journal of Molecular Sciences. 2020; 21(18):6922. https://doi.org/10.3390/ijms21186922
Chicago/Turabian StyleTamura, Yasuhisa, Kumi Takata, Asami Eguchi, and Yosky Kataoka. 2020. "Selective Elimination of NG2-Expressing Hair Follicle Stem Cells Exacerbates the Sensitization Phase of Contact Dermatitis in a Transgenic Rat Model" International Journal of Molecular Sciences 21, no. 18: 6922. https://doi.org/10.3390/ijms21186922