The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea
Abstract
:1. Introduction
2. Results
2.1. Analysis of Bcser1 and Bcser2 in B. cinerea
2.2. Bcser2 Plays Important Roles in Sclerotial Formation and Conidiation
2.3. Bcser2 Is Essential for the Full Virulence of B. cinerea
2.4. Bcser2 Is Required for Infection Cushion Formation
3. Discussion
4. Materials and Methods
4.1. Fungal Strains and Growth Conditions
4.2. Bioinformatics Analysis
4.3. Gene Replacement and Complementation Strategy
4.4. Transformation of B. cinerea
4.5. Molecular Analysis of B. cinerea Mutants
4.6. RNA Isolation and qRT-PCR
4.7. Virulence Assay
4.8. Stereomicroscopic Observation of Infection Cushions
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Liu, X.; Xie, J.; Fu, Y.; Jiang, D.; Chen, T.; Cheng, J. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. Int. J. Mol. Sci. 2020, 21, 603. https://doi.org/10.3390/ijms21020603
Liu X, Xie J, Fu Y, Jiang D, Chen T, Cheng J. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences. 2020; 21(2):603. https://doi.org/10.3390/ijms21020603
Chicago/Turabian StyleLiu, Xinqiang, Jiatao Xie, Yanping Fu, Daohong Jiang, Tao Chen, and Jiasen Cheng. 2020. "The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea" International Journal of Molecular Sciences 21, no. 2: 603. https://doi.org/10.3390/ijms21020603