Extracellular Matrix-Specific Platelet Activation Leads to a Differential Translational Response and Protein De Novo Synthesis in Human Platelets
Abstract
:1. Introduction
2. Results
2.1. De Novo Protein Synthesis of Activated Platelets Is Markedly Increased by Fibrinogen Binding
2.2. Adherent Platelet Morphology Is Specific to the Extracellular Matrix Stimulus
2.3. Platelet Activation Results in Polysomal Accumulation and Assembly of the Translational Machinery (Consisting of eIF4E, mRNAs and Vinculin)
2.4. Platelets Show a Differential and Stimulus-Dependent Protein Expression Profile after Exposure to Extracellular Matrix Proteins Laminin or Collagen and to Fibrinogen
2.5. Platelets That Are Adherent to Laminin Synthesize Proteins That Are Essential for Platelet Migration
3. Discussion
4. Material and Methods
4.1. Platelet Isolation
4.2. Metabolic Radiolabeling of Human Platelet Proteins
4.3. Two-Dimensional Electrophoresis
4.4. Immunoblotting
4.5. Confocal Microscopy
4.6. Polysome Analysis
4.7. cDNA Arrays
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Protein Spot # | Protein Identified |
---|---|
spot #1055 | Tubulin beta-1 chain |
spot #1090 | Isoform 2 of gelsolin |
spot #1091 | Isoform 2 of gelsolin |
spot #1242 | FERMT3 Isoform 2 of Fermitin family homolog 3 |
spot #1245 | FERMT3 Isoform 2 of Fermitin family homolog |
Transaldolase (TALDO1) | |
spot #1462 | CAPZB cDNA FLJ60094, highly similar to F-actin |
capping protein subunit beta | |
spot #1532 | FGA Isoform 1 of Fibrinogen alpha chain |
spot #1799 | CAP1 Isoform 1 of Adenylyl cyclase-associated protein1 |
HK1 Isoform 3 of Hexokinase-1 | |
spot #478 | VCL Isoform 1 of Vinculin |
TLN1 Talin-1 | |
ACTN1 Alpha-actinin-1 | |
FGG Isoform Gamma-B of Fibrinogen gamma chain | |
ACTN4 Alpha-actinin-4 | |
spot #479 | VCL Isoform 1 of Vinculin |
TLN1 Talin-1 | |
ACTN1 Alpha-actinin-1 | |
FGG Isoform Gamma-B of Fibrinogen gamma chain | |
ACTN4 Alpha-actinin-4 | |
spot #562 | TLN1 Talin-1 |
FLNA Isoform 2 of Filamin-A | |
TLN2 Talin-2 | |
spot #585 | VCL Isoform 1 of Vinculin |
TLN1 Talin-1 | |
GSN Isoform 2 of Gelsolin | |
spot #588 | VCL Isoform 1 of Vinculin |
GSN Isoform 2 of Gelsolin | |
spot #6083 | VCL Isoform 1 of Vinculin |
spot #961 | CORO1C Coronin-1C |
spot #997 | CORO1A Coronin-1A |
ENO1 Isoform alpha-enolase of alpha-enolase |
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Kraemer, B.F.; Geimer, M.; Franz-Wachtel, M.; Lamkemeyer, T.; Mannell, H.; Lindemann, S. Extracellular Matrix-Specific Platelet Activation Leads to a Differential Translational Response and Protein De Novo Synthesis in Human Platelets. Int. J. Mol. Sci. 2020, 21, 8155. https://doi.org/10.3390/ijms21218155
Kraemer BF, Geimer M, Franz-Wachtel M, Lamkemeyer T, Mannell H, Lindemann S. Extracellular Matrix-Specific Platelet Activation Leads to a Differential Translational Response and Protein De Novo Synthesis in Human Platelets. International Journal of Molecular Sciences. 2020; 21(21):8155. https://doi.org/10.3390/ijms21218155
Chicago/Turabian StyleKraemer, Bjoern F., Marc Geimer, Mirita Franz-Wachtel, Tobias Lamkemeyer, Hanna Mannell, and Stephan Lindemann. 2020. "Extracellular Matrix-Specific Platelet Activation Leads to a Differential Translational Response and Protein De Novo Synthesis in Human Platelets" International Journal of Molecular Sciences 21, no. 21: 8155. https://doi.org/10.3390/ijms21218155