Seroconversion and Skin Mucosal Parameters during Koi Herpesvirus Shedding in Common Carp, Cyprinus carpio
Round 1
Reviewer 1 Report
In the first experiment, researchers aimed to investigate the effects of non-permissive temperature (12°C) or permissive temperatures (17 and 22°C) on seroconversion and skin mucosal parameters of common carp, Cyprinus carpio exposed to koi herpesvirus. Then, the survivors of the initial experiment were exposed to temperature increase to 22°C 500 D.
The topic of this study is innovative and interesting, where the researchers studied the role of the seroconversion on the viral reactivation in the second experiment.
The experiments are well designed and performed. The paper is well written.
The introduction is ok and properly reviewed the relevant literature.
The methods used to perform the study are clear and adequate.
The results presented are adequate.
The tables and figures used to show them are adequate.
The discussion is fairly comprehensive.
The conclusion was supported by the results and clearly expressed the main hypothesis of the study.
Therefore, I think the current version of the paper is suitable to publish in the "International Journal of Molecular Sciences".
Reviewer 2 Report
This manuscript by Cano et al. presents the gene expression of key mucosal parameters during koi herpesvirus infection at permissive and non-permissive temperatures. And the examined animals were subjected to temperature increase (heat stress to 22 °C) for the role of the seroconversion on the viral reactivation.
The common carp (Crypinus carpio Linnaeus, 1758) is a freshwater fish species with wide range of the World. Koi herpesvirus disease observed worldwide due to the intense global trade of koi and common carp. Since 2006, koi herpesvirus disease became a reportable disease to the Office International des Epizooties.
The manuscript contains new scientific results and the authors interpreted it very well. These information will be useful for researchers working on same topic. The manuscript is generally written OK and it is a very good paper of an experienced team.
Congratulations for this study. The manuscript contains new scientific results and the authors interpreted it very well.
