Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology
Abstract
:1. Introduction
2. Results
2.1. O2•− and H2O2 Detection by Tissue Printing
2.2. In Situ Localization of O2•− and H2O2 by Histochemical Staining
2.3. O2•− and H2O2 Quantification by ImageJ
2.4. O2•− and H2O2 Quantification by Flow Cytometry
2.5. O2•− and H2O2 Quantification by Spectrophotometric Method
3. Discussion
4. Materials and Methods
4.1. Seed Material
4.2. O2•− and H2O2 Localization by NBT- and DAB-Mediated Tissue Printing and Content Determination by ImageJ
4.3. In Situ Localization and Level of O2•− and H2O2 and Content Determination by ImageJ
4.4. Determination of O2•− and H2O2 Content by Flow Cytometry
4.5. Determination of O2•− and H2O2 Content by Spectrophotometric Method
4.6. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CDCDHFDA | 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate di(acetoxymethyl ester) |
DAB | 3,3′-diaminobenzidine |
DHE | dihydroethidium |
DPI | Diphenyleneiodonium |
FCM | Flow cytometry |
NADPH oxidase | Nicotinamide adenine dinucleotide phosphate oxidase |
NBT | Nitro blue tetrazolium |
PCD | Programmed cell death |
ROS | Reactive Oxygen Species |
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Cembrowska-Lech, D. Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology. Int. J. Mol. Sci. 2020, 21, 8656. https://doi.org/10.3390/ijms21228656
Cembrowska-Lech D. Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology. International Journal of Molecular Sciences. 2020; 21(22):8656. https://doi.org/10.3390/ijms21228656
Chicago/Turabian StyleCembrowska-Lech, Danuta. 2020. "Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology" International Journal of Molecular Sciences 21, no. 22: 8656. https://doi.org/10.3390/ijms21228656