The Role of Copper (II) on Kininogen Binding to Tropomyosin in the Presence of a Histidine–Proline-Rich Peptide
Abstract
:1. Introduction
2. Results
2.1. Tropomyosin/HKa Binding: In Presence of TetraHPRG, Cu2+ or Zn2+
2.2. pH Effect
2.3. TetraHPRG Effect in “Pre-Treatment”
3. Discussion
4. Materials and Methods
4.1. Proteins, Peptides, and Materials
4.2. ELISA Assay
4.3. CD Spectroscopy
4.4. Potentiometric Titrations
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Log Beta |
---|---|
1 1 10 | 71.65 (4) |
1 1 6 | 51.08 (6) |
1 1 4 | 39.98 (4) |
1 1 2 | 23.44 (5) |
1 1 0 | 4.45 (6) |
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Santoro, A.M.; Zimbone, S.; Magrì, A.; La Mendola, D.; Grasso, G. The Role of Copper (II) on Kininogen Binding to Tropomyosin in the Presence of a Histidine–Proline-Rich Peptide. Int. J. Mol. Sci. 2020, 21, 9343. https://doi.org/10.3390/ijms21249343
Santoro AM, Zimbone S, Magrì A, La Mendola D, Grasso G. The Role of Copper (II) on Kininogen Binding to Tropomyosin in the Presence of a Histidine–Proline-Rich Peptide. International Journal of Molecular Sciences. 2020; 21(24):9343. https://doi.org/10.3390/ijms21249343
Chicago/Turabian StyleSantoro, Anna Maria, Stefania Zimbone, Antonio Magrì, Diego La Mendola, and Giulia Grasso. 2020. "The Role of Copper (II) on Kininogen Binding to Tropomyosin in the Presence of a Histidine–Proline-Rich Peptide" International Journal of Molecular Sciences 21, no. 24: 9343. https://doi.org/10.3390/ijms21249343