Efficient Prodrug Activator Gene Therapy by Retroviral Replicating Vectors Prolongs Survival in an Immune-Competent Intracerebral Glioma Model
Abstract
:1. Introduction
2. Results
2.1. GALV-RRV Achieves Highly Efficient Transgene Delivery to Glioma Cells
2.2. Efficient and Progressive Spread of GALV-RRV in an Intracerebral Glioma Model
2.3. No Detectable Spread of GALV-RRV to Extratumoral Tissues
2.4. Dose-Dependent Cytotoxicity of RRV-Transduced Cells after Prodrug Administration
2.5. RRV-Mediated Prodrug Activator Gene Delivery Significantly Improves Survival of Immune-Competent Rats Bearing Gliomas
2.6. MicroPET Imaging of RG-2 Glioma-Bearing Rats after Prodrug Activator Gene Therapy
3. Discussion
4. Materials and Methods
4.1. Viral Vectors and Cell Lines
4.2. Viral Vector Replication Assays in Glioma Cells
4.3. Viral Vector Replication Assay in Intracerebral Glioma Model
4.4. Real-Time PCR Analysis
4.5. In Vitro Cytotoxicity Assay
4.6. Survival Assay Using Intracerebral Glioma Models
4.7. MicroPET Imaging
4.8. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Copies of GALV env Per Cell | |
---|---|
tumor | 1.46 ± 0.23 |
contralateral normal brain | − |
bone marrow | − |
spleen | − |
intestine | − |
liver | − |
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Chen, S.-H.; Sun, J.-M.; Chen, B.-M.; Lin, S.-C.; Chang, H.-F.; Collins, S.; Chang, D.; Wu, S.-F.; Lu, Y.-C.; Wang, W.; et al. Efficient Prodrug Activator Gene Therapy by Retroviral Replicating Vectors Prolongs Survival in an Immune-Competent Intracerebral Glioma Model. Int. J. Mol. Sci. 2020, 21, 1433. https://doi.org/10.3390/ijms21041433
Chen S-H, Sun J-M, Chen B-M, Lin S-C, Chang H-F, Collins S, Chang D, Wu S-F, Lu Y-C, Wang W, et al. Efficient Prodrug Activator Gene Therapy by Retroviral Replicating Vectors Prolongs Survival in an Immune-Competent Intracerebral Glioma Model. International Journal of Molecular Sciences. 2020; 21(4):1433. https://doi.org/10.3390/ijms21041433
Chicago/Turabian StyleChen, Shih-Han, Jui-Ming Sun, Bing-Mao Chen, Sheng-Che Lin, Hao-Fang Chang, Sara Collins, Deching Chang, Shu-Fen Wu, Yin-Che Lu, Weijun Wang, and et al. 2020. "Efficient Prodrug Activator Gene Therapy by Retroviral Replicating Vectors Prolongs Survival in an Immune-Competent Intracerebral Glioma Model" International Journal of Molecular Sciences 21, no. 4: 1433. https://doi.org/10.3390/ijms21041433