In Situ Profiling of the Three Dominant Phyla Within the Human Gut Using TaqMan PCR for Pre-Hospital Diagnosis of Gut Dysbiosis
, Huy Quang Pham 1, YeonGyun Jung 1, Jerald Conrad Ibal 1, SeungDae Choi 1, Gi-Ung Kang 1, Sowon Park 2, Yunkoo Kang 2, Seung Kim 2, Hong Koh 2 and Jae-Ho Shin 1,*Round 1
Reviewer 1 Report
This manuscript reported the profiling of the three dominant phyla within the human gut using TaqMan PCR for pre-hospital diagnosis of gut dysbiosis. The result showed MTq-PCR assay in this work may be a practical microbiota profiling alternative for diagnosing and monitoring gut dysbiosis in UC patients during emergency situations. Overall, the work was well conceived and designed. The quality of figures should be improved before the publication.
Author Response
We appreciate this recommendation. We totally updated all the figures with efforts and hope we have improved them (Please check Figures 1~6).
Reviewer 2 Report
It is indeed a very interesting technology and as well in comparing the rapid and less expensive TaqMan PCR methodology with NGS procedure. I am only wondering why the healthy patients N=10 and UC N=6. They could have done equal numbers by adding another four UC samples. Overhaul, it is a great methodology and team work.
Author Response
We appreciate the reviewer for the comment. We only got six samples from hospital during experimental period. More importantly, we didn’t carefully think about that issue when we plan the experiment because the main focus of the study was not to compare the UC patients and healthy individuals but rather for comparing the two methods, MTq-PCR and NGS. We tried to update and redraw the figures 3 and 4 for more clear view.
