Thymosin β4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model
Abstract
:1. Introduction
2. Results
2.1. Treatment with Tβ4 Promotes Morphological Changes in hASCs
2.2. Tβ4 Induces the Active State of hASCs
2.3. Additional Treatment with Tβ4 Enhances Endothelial Differentiation of hASCs
2.4. Transplantation of hASCs in Combination with Tβ4 Improves Blood Vessel Recruitment and Blood Flow After Ischemia
3. Discussion
4. Materials and Methods
4.1. Cell Source
4.2. Real-Time Polymerase Chain Reaction
4.3. Immunofluorescence Staining
4.4. Western Blot Analysis
4.5. Wound Healing Assay
4.6. Microbead Sprouting Assay
4.7. Induction of Endothelial Differentiation
4.8. Dorsal Window Chamber Assay
4.9. Mouse Hindlimb Ischemia Model
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kim, J.-H.; Lim, I.-R.; Park, C.-Y.; Joo, H.J.; Noh, J.-M.; Choi, S.-C.; Hong, S.J.; Lim, D.-S. Thymosin β4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model. Int. J. Mol. Sci. 2020, 21, 2166. https://doi.org/10.3390/ijms21062166
Kim J-H, Lim I-R, Park C-Y, Joo HJ, Noh J-M, Choi S-C, Hong SJ, Lim D-S. Thymosin β4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model. International Journal of Molecular Sciences. 2020; 21(6):2166. https://doi.org/10.3390/ijms21062166
Chicago/Turabian StyleKim, Jong-Ho, I-Rang Lim, Chi-Yeon Park, Hyung Joon Joo, Ji-Min Noh, Seung-Cheol Choi, Soon Jun Hong, and Do-Sun Lim. 2020. "Thymosin β4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model" International Journal of Molecular Sciences 21, no. 6: 2166. https://doi.org/10.3390/ijms21062166