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Volume 21
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Article

Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain

by
Eleonora Persoons
1,2,
Katrien De Clercq
1,2,
Charlotte Van den Eynde
1,2,
Sílvia João Poseiro coutinho Pinto
2,
Katrien Luyten
1,
Rita Van Bree
1,
Carla Tomassetti
1,3,
Thomas Voets
2 and
Joris Vriens
1,*
1
Laboratory of Endometrium, Endometriosis & Reproductive Medicine, Department of Development and Regeneration, KU Leuven, Herestraat 49 box 611, 3000 Leuven, Belgium
2
Laboratory of Ion Channel Research, Department of Cellular and Molecular Medicine, KU Leuven, VIB Center for Brain & Disease Research, Herestraat 49 box 802, 3000 Leuven, Belgium
3
Leuven University Fertility Center, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2326; https://doi.org/10.3390/ijms21072326
Submission received: 28 February 2020 / Revised: 23 March 2020 / Accepted: 25 March 2020 / Published: 27 March 2020
(This article belongs to the Special Issue Molecular Research of Endometrial Pathophysiology 2.0)
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Abstract

Endometriosis is a prevalent gynecologic disease, defined by dysfunctional endometrium-like lesions outside of the uterine cavity. These lesions are presumably established via retrograde menstruation, i.e., endometrial tissue that flows backwards during menses into the abdomen and deposits on the organs. As ongoing pain is one of the main pain symptoms of patients, an animal model that illuminates this problem is highly anticipated. In the present study, we developed and validated a rat model for ongoing endometriosis-associated pain. First, menstrual endometrial tissue was successfully generated in donor rats, as validated by gross examination, histology and qPCR. Next, endometriosis was induced in recipient animals by intraperitoneal injection of menstrual tissue. This resulted in neuro-angiogenesis as well as established endometriosis lesions, which were similar to their human counterparts, since epithelial and stromal cells were observed. Furthermore, significant differences were noted between control and endometriosis animals concerning bodyweight and posture changes, indicating the presence of ongoing pain in animals with endometriosis. In summary, a rat model for endometriosis was established that reliably mimics the human pathophysiology of endometriosis and in which signs of ongoing pain were detected, thus providing a new research tool for therapy development.
Keywords: endometriosis; menstrual tissue; menstruating rat; ongoing pain endometriosis; menstrual tissue; menstruating rat; ongoing pain

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MDPI and ACS Style

Persoons, E.; De Clercq, K.; Van den Eynde, C.; Pinto, S.J.P.c.; Luyten, K.; Van Bree, R.; Tomassetti, C.; Voets, T.; Vriens, J. Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain. Int. J. Mol. Sci. 2020, 21, 2326. https://doi.org/10.3390/ijms21072326

AMA Style

Persoons E, De Clercq K, Van den Eynde C, Pinto SJPc, Luyten K, Van Bree R, Tomassetti C, Voets T, Vriens J. Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain. International Journal of Molecular Sciences. 2020; 21(7):2326. https://doi.org/10.3390/ijms21072326

Chicago/Turabian Style

Persoons, Eleonora, Katrien De Clercq, Charlotte Van den Eynde, Sílvia João Poseiro coutinho Pinto, Katrien Luyten, Rita Van Bree, Carla Tomassetti, Thomas Voets, and Joris Vriens. 2020. "Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain" International Journal of Molecular Sciences 21, no. 7: 2326. https://doi.org/10.3390/ijms21072326

APA Style

Persoons, E., De Clercq, K., Van den Eynde, C., Pinto, S. J. P. c., Luyten, K., Van Bree, R., Tomassetti, C., Voets, T., & Vriens, J. (2020). Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain. International Journal of Molecular Sciences, 21(7), 2326. https://doi.org/10.3390/ijms21072326

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