Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. Effects of Short Hypergravity Treatments
2.2. Effect of Longer Hypergravity Treatments
3. Discussion
4. Materials and Methods
4.1. Cell line and Culture
4.2. Reagents
4.3. Hypergravity Protocols
4.4. Wound Healing (Scratch) Assay
4.5. Tube Formation Assay
4.6. Antibodies, Cell Staining and Fluorescence Analysis
4.7. Cell Fixation and RNA Extraction
4.8. RT2 Profiler PCR Arrays
4.9. Statistical Analysis
Supplementary Materials
Supplementary File 1Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
Anova | Analysis of Variance |
B2M | Beta-2-Microglobulin |
BAEC | Bovine Aortic Endothelial Cells |
DAPI | 4′,6-diamidino-2-phenylindole |
EC | Endothelial Cells |
ESA | European Space Agency |
ESTEC | European Space Research and Technology Centre |
FAK | Focal Adhesion Kinase |
HMEC-1 | human microvascular endothelial cells 1 |
HPRT1 | Hypoxanthine Phosphoribosyltransferase 1 |
HUVEC | human umbilical vein endothelial cells |
ICAM1 | Intercellular Adhesion Molecule 1 |
IF | intermediate filaments |
ISS | International Space Station |
LDC | Large Diameter Centrifuge |
NO | Nitric Oxide |
NOS | Nitric Oxide Synthase |
PCR | Polymerase Chain Reaction |
PROCR | Protein C Receptor |
RPL0 | Ribosomal Protein Lateral Stalk Subunit P0 |
RPM | Random Positioning Machines |
RT | room temperature |
SANS | space flight-associated neuro-ocular syndrome |
SEM | standard error of the mean |
SYT | Spin Your Thesis |
YAP | Yes-Associated Protein 1 |
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4g Vs. Ctr | 20g Vs. Ctr | 20g Vs. 4g | |
---|---|---|---|
Up | FASLG, NPPB, PLG. | TNFSF10, IL1β, ICAM1, TGFB1, MMP2, CFLAR, APOE, COL18A1, FN1, NOS3, PLAU, VWF. | ENG, MMP1, PLAT, PROCR, SERPINE1, TEK, TFPI, TGFB1, VWF, ACTB, GAPDH, NOS3. |
Down | VWF | THBD |
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De Cesari, C.; Barravecchia, I.; Pyankova, O.V.; Vezza, M.; Germani, M.M.; Scebba, F.; van Loon, J.J.W.A.; Angeloni, D. Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells. Int. J. Mol. Sci. 2020, 21, 2354. https://doi.org/10.3390/ijms21072354
De Cesari C, Barravecchia I, Pyankova OV, Vezza M, Germani MM, Scebba F, van Loon JJWA, Angeloni D. Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells. International Journal of Molecular Sciences. 2020; 21(7):2354. https://doi.org/10.3390/ijms21072354
Chicago/Turabian StyleDe Cesari, Chiara, Ivana Barravecchia, Olga V. Pyankova, Matteo Vezza, Marco M. Germani, Francesca Scebba, Jack J. W. A. van Loon, and Debora Angeloni. 2020. "Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells" International Journal of Molecular Sciences 21, no. 7: 2354. https://doi.org/10.3390/ijms21072354