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Article

The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer

by
Felix Grambow
1,*,
Rico Rutkowski
2,†,
Fred Podmelle
1,†,
Katrin Schmoeckel
3,
Florian Siegerist
4,
Grzegorz Domanski
5,
Matthias W. Schuster
1 and
Grazyna Domanska
3
1
Department of Oral and Maxillofacial Surgery/Plastic Surgery, University Medicine Greifswald, 17489 Greifswald, Germany
2
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg Eppendorf, 20251 Hamburg, Germany
3
Department of Immunology, University Medicine Greifswald, 17489 Greifswald, Germany
4
Department of Anatomy and Cell Biology, University Medicine Greifswald, 17489 Greifswald, Germany
5
Department of Neonatology and Pediatric Intensive Care, University Medicine Greifswald, 17489 Greifswald, Germany
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(8), 2869; https://doi.org/10.3390/ijms21082869
Submission received: 24 March 2020 / Revised: 15 April 2020 / Accepted: 17 April 2020 / Published: 20 April 2020
(This article belongs to the Special Issue Optimization of Biomaterials for Reconstructive and Regenerative Medicine)
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Abstract

The local anesthetic lidocaine, which has been used extensively during liposuction, has been reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem cells (ASCs), preadipocytes, mature adipocytes, and leukocytes in the fatty and fluid portion of the lipoaspirate using antibody staining and flow cytometry analyses. Adipose tissue was harvested from 11 female patients who underwent liposuction. Abdominal subcutaneous fat tissue was infiltrated with tumescent local anesthesia, containing lidocaine on the left and lacking lidocaine on the right side of the abdomen, and harvested subsequently. Lidocaine had no influence on the relative distribution, cell number, or viability of ASCs, preadipocytes, mature adipocytes, or leukocytes in the stromal-vascular fraction. Assessing the fatty and fluid portions of the lipoaspirate, the fatty portions contained significantly more ASCs (p < 0.05), stem cells expressing the preadipocyte marker Pref-1 (p < 0.01 w/lidocaine, p < 0.05 w/o lidocaine), and mature adipocytes (p < 0.05 w/lidocaine, p < 0.01 w/o lidocaine) than the fluid portions. Only the fatty portion should be used for transplantation. This study found no evidence that would contraindicate the use of lidocaine in lipotransfer. Limitations of the study include the small sample size and the inclusion of only female patients.
Keywords: lipofilling; autologous lipotransfer; adipose-derived stem cells; lidocaine lipofilling; autologous lipotransfer; adipose-derived stem cells; lidocaine

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MDPI and ACS Style

Grambow, F.; Rutkowski, R.; Podmelle, F.; Schmoeckel, K.; Siegerist, F.; Domanski, G.; Schuster, M.W.; Domanska, G. The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer. Int. J. Mol. Sci. 2020, 21, 2869. https://doi.org/10.3390/ijms21082869

AMA Style

Grambow F, Rutkowski R, Podmelle F, Schmoeckel K, Siegerist F, Domanski G, Schuster MW, Domanska G. The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer. International Journal of Molecular Sciences. 2020; 21(8):2869. https://doi.org/10.3390/ijms21082869

Chicago/Turabian Style

Grambow, Felix, Rico Rutkowski, Fred Podmelle, Katrin Schmoeckel, Florian Siegerist, Grzegorz Domanski, Matthias W. Schuster, and Grazyna Domanska. 2020. "The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer" International Journal of Molecular Sciences 21, no. 8: 2869. https://doi.org/10.3390/ijms21082869

APA Style

Grambow, F., Rutkowski, R., Podmelle, F., Schmoeckel, K., Siegerist, F., Domanski, G., Schuster, M. W., & Domanska, G. (2020). The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer. International Journal of Molecular Sciences, 21(8), 2869. https://doi.org/10.3390/ijms21082869

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