Increase of the Intracellular Zinc Concentration Leads to an Activation and Internalisation of the Epidermal Growth Factor Receptor in A549 Cells
Abstract
:1. Introduction
2. Results
2.1. Zinc and Pyrithione as Well as SNOC Increase the Intracellular Zinc Concentration
2.2. Increase of the Free Intracellular Zinc Level Activates the EGFR
2.3. Increase of the Intracellular Zinc Level Provokes EGFR Internalisation
2.4. EGF Stimulation Does Not Increase the Intracellular Zinc Concentration
2.5. Simultaneous Stimulation Promotes Activation and Internalisation of the EGFR
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Cell Stimulation
4.2. Measurement of the Free Intracellular Zinc Concentration
4.3. Sample Preparation and Western Blotting
4.4. Measurement of EGFR Surface Expression
4.5. Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
EGF | Epidermal growth factor |
EGFR | Epidermal growth factor receptor |
SNOC | S-nitrosocysteine |
SNAP | S-nitroso-N-acetylpenicillamine |
NO | Nitric oxide |
Zn | Zinc |
Pyr | Pyrithione |
TPEN | N,N,N,N-tetrakis(2-pyridylmethyl)-ethylenediamine |
BSA | Bovine serum albumin |
PBS | Phosphate-buffered saline |
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Barth, L.-M.; Rink, L.; Wessels, I. Increase of the Intracellular Zinc Concentration Leads to an Activation and Internalisation of the Epidermal Growth Factor Receptor in A549 Cells. Int. J. Mol. Sci. 2021, 22, 326. https://doi.org/10.3390/ijms22010326
Barth L-M, Rink L, Wessels I. Increase of the Intracellular Zinc Concentration Leads to an Activation and Internalisation of the Epidermal Growth Factor Receptor in A549 Cells. International Journal of Molecular Sciences. 2021; 22(1):326. https://doi.org/10.3390/ijms22010326
Chicago/Turabian StyleBarth, Lisa-Marie, Lothar Rink, and Inga Wessels. 2021. "Increase of the Intracellular Zinc Concentration Leads to an Activation and Internalisation of the Epidermal Growth Factor Receptor in A549 Cells" International Journal of Molecular Sciences 22, no. 1: 326. https://doi.org/10.3390/ijms22010326