Circulating Exosomal miRNAs as Biomarkers for the Diagnosis and Prognosis of Colorectal Cancer
Abstract
:1. Introduction
2. Results
2.1. Exosomal miRNAs for the Screening and Diagnosis of CRC
2.2. Exosomal miRNAs for Estimating Prognosis in Resected Patients
2.3. Exosomal miRNAs for Surveillance and Follow-up
2.4. Exosomal miRNAs for the Management of Metastatic Disease
3. Discussion
4. Materials and Methods
4.1. Bibliographic Search Strategy
4.2. Selection of Studies
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Authors | Sample Type | Sample Processing Conditions | Method of Exosome Isolation | Method of RNA/miRNA Isolation | Method of miRNA Detection |
---|---|---|---|---|---|
Karimi et al., 2019 | Serum | <2 hours of collection; 2000× g centrifugation for 10 min | ExoQuick (System Biosciences) | Not described | qRT-PCR |
Liu et al., 2018 | Plasma | Not described | Invitrogen Total Exosome Isolation Kit (Invitrogen) | miRNeasy Mini Kit (Qiagen) | qRT-PCR |
Ogata-Kawata et al., 2014 | Serum | Not described | Ultracentrifugation at 120,000× g for 70 min | Trizol LS reagent (Invitrogen) | Microarray and qRT-PCR |
Zhu et al., 2017 | Serum | Centrifugation at 1500 RPM for 10 min in 12 h after collection | ExoQuick Exosome Precipitation (System Biosciences) | mirVana Paris Kit ((Ambion) | qRT-PCR |
Ren et al., 2017 | Serum | Not described | exoRNeasy Serum/Plasma Max Kit | RNA Isolation Kit-miRNeasy Mini Kit | qRT-PCR |
Cheng et al., 2019 | Serum | Not described | Ultracentrifugation at 100,000× g for 120 min at 4 °C | Trizol reagent (Invitrogen) | qRT-PCR |
Wang et al., 2017 | Plasma | Centrifugation at 3000× g for 10 min 30 min after collection | ExoQuick Exosome Precipitation Solution (SBI) | miRNeasy Micro Kit (QIAGEN, Valencia, CA) | qRT-PCR |
Zhang et al., 2019 | Plasma | Centrifugation at 350 RCF for 10 min in 12 h | ExoQuick Exosome Precipitation Solution (System Biosciences) | mirVana Paris Kit (Ambion, Austin, TX, USA) | qRT-PCR |
Min et al., 2019 | Plasma | centrifugation at 3000× g for 15 min at 4°C | ultracentrifugation at 150,000× g, at 4 °C for 4 h | miRNeasy® Mini kit (Qiagen) | qRT-PCR |
Liu et al., 2018 | Plasma | Not described | Not described | Trizol LS Reagent (Invitrogen, CA, USA) | qRT-PCR |
Takano et al., 2017 | Serum | Centrifugation at 3000 RPM for 10 min at 4°C | Ultracentrifugation at 100,000× g for 70 min at 4 °C | miRNeasy serum/plasma kit (Qiagen) | qRT-CR |
Yan et al., 2017 | Serum | Centrifugation at 2000 RPM for 10 min | Total Exosome Isolation Kit (Invitrogen) | Trizol LS (Invitrogen) and miRNeasy mini kit (Qiagen) | Microarray and qRT-PCR |
Yan et al., 2018 | Serum | Centrifugation at 1200× g for 10 min at 4 °C | Invitrogen™ Total Exosome Isolation Kit | miRNeasy mini kit (Qiagen) | qRT-PCR |
Yan et al., 2018 | Serum | Not described | Total exosome isolation kit (Invitrogen) | Trizol reagent (Invitrogen) | qRT-PCR |
Fu et al., 2018 | Serum | Centrifugation at 1500 RPM for 10 min, and centrifugation at 12,000 RPM for 2 min within 12 h of collection | Exosome Isolation Reagent RiboTM | HiPure Liquid RNA/miRNA Kit (Magen, China) | qRT-PCR |
Peng; Gu; Yan., 2019 | Serum | Centrifugation at 1500–2000 RPM for 20 min | Total exosome isolation kit (Invitrogen) | Trizol reagent (Invitrogen miRNeasy mini kit (Qiagen) | qRT-CR |
Tang et al., 2019 | Serum | Centrifugation at 10,000× g at 4 °C for 30 min | Ultracentrifugation at 100,000× g for 120 min at 4 °C | Trizol reagent | qRT-PCR |
Yu et al., 2017 | Serum | Centrifugation at 3000× g for 15 min at 4 °C | ExoQuick Exosome Precipitation Solution (SBI) | MirVana microRNA isolation kit (Life technologies) | qRT-CR |
Tsukamoto et al., 2017 | Plasma | Centrifugation at 1200× g for 10 min at 4 °C | Ultracentrifugation at 15,000× g for 70 min at 4 °C | miRNeasy Serum/Plasma Kit (Qiagen, Hilden, Germany) | Microarray and qRT-PCR |
Shao et al., 2018 | Plasma | Not described | Ultracentrifugation at 160,000× g for 16 h | tCLN biochip | tCLN biochip |
Santasusagna et al., 2018 | Plasma | Centrifugation at 5000× g for 10 min | Ultracentrifugation | miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) | qRT-PCR |
Matsumura et al., 2015 | Serum | Not described | Total Exosome Isolation Kit (Invitrogen) | miRNeasy mini kit (Qiagen) | Microarray and qRT-PCR |
Liu et al., 2016 | Serum | Centrifugation at 3000× g for 10 min at ambient temperature immediately after blood collection | ExoQuick kit (SBI) | SeraMir exosome RNA kit (SBI) | RNA-Seq and qRT-PCR |
Li et al., 2017 | Plasma | Centrifugation at 3000× g for 5 min at 4 °C | ExoCapTM Exosome Isolation and Enrichment kit (JSR) | Trizol reagent (Invitrogen, Carlsbad, CA, USA) | Flow cytometry test and qRT-PCR |
Monzo et al., 2017 | Plasma | Centrifugation at 5000× g for 10 min | Ultracentrifugation | miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) | qRT-PCR |
Bjørnetrø et al., 2019 | Plasma | Centrifugation at 2000× g for 10 min | miRCURY™ Exosome Isolation Kit (Exiqon) | miRNeasy (Qiagen, Hilden, Germany) | qRT-PCR |
Jin et al., 2019 | Serum | Centrifugation at 2000 RPM for 10 min | Total Exosome Isolation Kit (Invitrogen) | Trizol Reagent (Invitrogen, Carlsbad, CA) | qRT-PCR |
Yagi et al., 2019 | Plasma | Centrifugation at 1200 × g for 10 min at 4 °C | Ultracentrifugation at 100,000× g for 70 min at 4 °C | miRNeasy serum/plasma kit (Qiagen, Hilden, Germany) | Microarray and qRT-PCR |
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Alves dos Santos, K.; Clemente dos Santos, I.C.; Santos Silva, C.; Gomes Ribeiro, H.; de Farias Domingos, I.; Nogueira Silbiger, V. Circulating Exosomal miRNAs as Biomarkers for the Diagnosis and Prognosis of Colorectal Cancer. Int. J. Mol. Sci. 2021, 22, 346. https://doi.org/10.3390/ijms22010346
Alves dos Santos K, Clemente dos Santos IC, Santos Silva C, Gomes Ribeiro H, de Farias Domingos I, Nogueira Silbiger V. Circulating Exosomal miRNAs as Biomarkers for the Diagnosis and Prognosis of Colorectal Cancer. International Journal of Molecular Sciences. 2021; 22(1):346. https://doi.org/10.3390/ijms22010346
Chicago/Turabian StyleAlves dos Santos, Katiusse, Isabelle Cristina Clemente dos Santos, Carollyne Santos Silva, Hériks Gomes Ribeiro, Igor de Farias Domingos, and Vivian Nogueira Silbiger. 2021. "Circulating Exosomal miRNAs as Biomarkers for the Diagnosis and Prognosis of Colorectal Cancer" International Journal of Molecular Sciences 22, no. 1: 346. https://doi.org/10.3390/ijms22010346