Osteogenesis of 3D-Printed PCL/TCP/bdECM Scaffold Using Adipose-Derived Stem Cells Aggregates; An Experimental Study in the Canine Mandible
Abstract
:1. Introduction
2. Results
2.1. Outcomes of the Evaluation Using CT
CT Results: Coronal, Axial, and Sagittal Views
2.2. Histological Findings
2.3. RT–PCR
2.4. Western Blot
3. Discussion
4. Materials and Methods
4.1. Materials
4.1.1. 3D Printing Scaffold Manufacturing of PCL/TCP/bdECM
4.1.2. Identification of ADSCs and Aggregates Formation
4.1.3. Experimental Animals and Groups
4.2. Experimental Methods
4.3. Outcome Evaluation
4.3.1. Evaluation Using CT
4.3.2. Histological Evaluation
4.3.3. RT–PCR
4.3.4. Western Blot
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Sequence (5′-3′) |
---|---|
COL1-dog-F | CTCGTCACAGTTGGGGTTGA |
COL1-dog-R | GGTGCAAGTATGAAGCGGGA |
OCN-dog-F | AATTGCGCTCGAGCATCTCT |
OCN-dog-R | ATTGCCACGGTTGCTACTGA |
RUNX2-dog-F | GGCGGCTATAACTCTTCCCA |
RUNX2-dog-R | ACGCAGCGGCTTTTTATTTCA |
GAPDH-F | GTCGGAGTCAACGGATTTGG |
GAPDH-R | GGGTGGAATCAATTGGAACAT |
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Lee, J.S.; Park, T.H.; Ryu, J.Y.; Kim, D.K.; Oh, E.J.; Kim, H.M.; Shim, J.-H.; Yun, W.-S.; Huh, J.B.; Moon, S.H.; et al. Osteogenesis of 3D-Printed PCL/TCP/bdECM Scaffold Using Adipose-Derived Stem Cells Aggregates; An Experimental Study in the Canine Mandible. Int. J. Mol. Sci. 2021, 22, 5409. https://doi.org/10.3390/ijms22115409
Lee JS, Park TH, Ryu JY, Kim DK, Oh EJ, Kim HM, Shim J-H, Yun W-S, Huh JB, Moon SH, et al. Osteogenesis of 3D-Printed PCL/TCP/bdECM Scaffold Using Adipose-Derived Stem Cells Aggregates; An Experimental Study in the Canine Mandible. International Journal of Molecular Sciences. 2021; 22(11):5409. https://doi.org/10.3390/ijms22115409
Chicago/Turabian StyleLee, Joon Seok, Tae Hyun Park, Jeong Yeop Ryu, Dong Kyu Kim, Eun Jung Oh, Hyun Mi Kim, Jin-Hyung Shim, Won-Soo Yun, Jung Bo Huh, Sung Hwan Moon, and et al. 2021. "Osteogenesis of 3D-Printed PCL/TCP/bdECM Scaffold Using Adipose-Derived Stem Cells Aggregates; An Experimental Study in the Canine Mandible" International Journal of Molecular Sciences 22, no. 11: 5409. https://doi.org/10.3390/ijms22115409