Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio
Abstract
:1. Introduction
2. Results
2.1. Egg Quality Indices
2.2. Image Analysis of 2D AUT × SDS PAGE
2.3. Histone Acetylation during In Vivo and In Vitro Oocyte Aging
2.4. Histone Acetyltransferase Activity during In Vivo and In Vitro Oocyte Aging
3. Discussion
4. Materials and Methods
4.1. Egg Storage and Quality Assessment
4.1.1. Experimental Fish Preparation
4.1.2. In Vivo and In Vitro Oocyte Aging
4.1.3. Artificial Fertilisation and Egg Developmental Success
4.2. Histone Modifications
4.2.1. Sample Collection for Histone Modification Analysis
4.2.2. Histone Isolation
4.2.3. Separation of Histone Modifications
4.2.4. Immunodetection of Specific Histone Acetylation
4.3. Histone Acetyltransferase Activity Assay
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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In Vivo Oocyte Aging | ||||||||||||||
HPO | 0 | 2 | 4 | 6 | 8 | 10 | 12 | 28 | ||||||
Eyeing % | 88 ± 9 a | 91 ± 5 a | 77 ± 16 ab | 66 ± 30 ab | 46 ± 25 bc | 15 ± 11 cd | 3 ± 4 d | 0 ± 0 d | ||||||
Hatching % | 83 ± 16 a | 88 ± 10 a | 71 ± 21 a | 59 ± 31 ab | 31 ± 19 bc | 6 ± 5 c | 0.7 ± 1 c | 0 ± 0 c | ||||||
In Vitro Oocyte Aging | ||||||||||||||
HPO | 0 | 2 | 4 | 6 | 8 | 10 | 12 | 28 | ||||||
Eyeing % | 95 ± 4 a | 94 ± 3 a | 92 ± 3 a | 84 ± 8 a | 62 ± 6 b | 40 ± 5 c | 0 ± 0 d | |||||||
Hatching % | 94 ± 4 a | 92 ± 1 a | 88 ± 5 a | 68 ± 15 ab | 36 ± 22 bc | 21 ± 9 cd | 0 ± 0 d |
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Waghmare, S.G.; Samarin, A.M.; Samarin, A.M.; Danielsen, M.; Møller, H.S.; Policar, T.; Linhart, O.; Dalsgaard, T.K. Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. Int. J. Mol. Sci. 2021, 22, 6036. https://doi.org/10.3390/ijms22116036
Waghmare SG, Samarin AM, Samarin AM, Danielsen M, Møller HS, Policar T, Linhart O, Dalsgaard TK. Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. International Journal of Molecular Sciences. 2021; 22(11):6036. https://doi.org/10.3390/ijms22116036
Chicago/Turabian StyleWaghmare, Swapnil Gorakh, Azin Mohagheghi Samarin, Azadeh Mohagheghi Samarin, Marianne Danielsen, Hanne Søndergård Møller, Tomáš Policar, Otomar Linhart, and Trine Kastrup Dalsgaard. 2021. "Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio" International Journal of Molecular Sciences 22, no. 11: 6036. https://doi.org/10.3390/ijms22116036