Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals
Abstract
:1. Introduction
2. Results
2.1. Differential Cell Counts
2.2. Proteomic Analysis
2.3. mRNA Expression
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Injections and Cell Preparation
4.3. Proteomic Analysis
4.3.1. Chemicals and Reagents
4.3.2. Protein Extraction and Quantification
4.3.3. On-Filter Tryptic Digestion of Proteins
4.3.4. NanoLC-MS/MS for Protein Identification
4.3.5. Mass Spectrometry Data Handling
4.4. Qualitative Data Analysis
4.5. RNA Extraction and mRNA Expression Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
α2M | Alfa-2-macroglobulin |
AMP | antimicrobial peptide |
βGBP | Beta-1,3-glucan binding protein |
proPO | prophenoloxidase |
SPH | serine protease homologue |
VMO1 | vitelline membrane outer layer 1 homologue |
References
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Accession Number | Protein Name | Fold Change, Log2ratio | p-Value (−log) |
---|---|---|---|
GBYW01031372.1 | Glutathione peroxidase 3 (GPX) | 2.708 | 14.95 |
GBYW01022610.1 GBYW01021121.1 GBYW01005009.1 | Heat shock cognate 71kDa protein | 2.374 | 17.72 |
GBYW01026927.1 | Trypsin-like serine protease | 2.170 | 15.740 |
GBYW01024713.1 | CLIP-domain serin protease | 2.088 | 5.28 |
GBYW01017647.1 GBYW01041647.1 | Flotillin-like protein | 2.041 | 9.49 |
GBYW01021864.1 | PDGF/VEGF domain protein | 2.031 | 7.41 |
GBYW01011929.1 | Peroxidase | 1.994 | 8.12 |
GBYW01037257.1 | Vitelline membrane outer layer protein 1 (VMO1) | 1.884 | 7.35 |
GBYW01020841.1 GBYW01022391.1 | Delta-1-pyrroline-5-carboxylate dehydrogenase | 1.845 | 14.50 |
GBYW01031650.1 | Unknown | 1.694 | 6.34 |
GBYW01028873.1 | i-type lysozyme | 1.673 | 8.474 |
GBYW01030110.1 GBYW01030111.1 | Unknown | 1.617 | 8.11 |
Accession Number | Protein Name | Biomarker Value | Fold Change Log2Ratio | p-Value |
---|---|---|---|---|
GBYW01037257.1 | VMO1 | 6.656 | 1.8836 | 0.006 |
EF523612.1 | Crustin 1 | 7.838 | 1.319 | 0.002 |
AY861652.1 | SPH1 | 5.418 | 1.404 | 0.0005 |
GBYW01031372.1 | Glutathione peroxidase (GPX) | 1.767 | 2.708 | 3,15199E-05 |
AY861653.1 | Mannose binding lectin | 5.133 | 1.1447 | 0.003 |
Y11145.2 | Masquerade | 7.177 | 0.652 | 0.003 |
GBYW01028010.1 | Kazal-type PI one domain | 5.682 | 0.949 | 0.009 |
GBYW01031855.1 | Kazal-type PI Agrin-like | 6.029 | 0.823 | 0.022 |
Proteins Upregulated | Accession Number | Proteins Upregulated | Accession Number |
---|---|---|---|
In Laminarin and Saline Injection Compared to Uninjected | In Laminarin Compared to Uninjected | ||
i-type lysozyme | GBYW01028873.1 | Glycine-rich peptide | GBYW01011676.1 |
Crustin -like AMP | GBYW01028095.1 | Kazal-type | GBYW01031549.1 |
Masquerade | Y11145.2 | Putative chitin binding protein | GBYW01037126.1 |
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Ekblom, C.; Söderhäll, K.; Söderhäll, I. Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals. Int. J. Mol. Sci. 2021, 22, 6464. https://doi.org/10.3390/ijms22126464
Ekblom C, Söderhäll K, Söderhäll I. Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals. International Journal of Molecular Sciences. 2021; 22(12):6464. https://doi.org/10.3390/ijms22126464
Chicago/Turabian StyleEkblom, Charlotta, Kenneth Söderhäll, and Irene Söderhäll. 2021. "Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals" International Journal of Molecular Sciences 22, no. 12: 6464. https://doi.org/10.3390/ijms22126464