Expression Profiling and Bioinformatics Analysis of CircRNA in Mice Brain Infected with Rabies Virus
Abstract
:1. Introduction
2. Results
2.1. Validation of RABV Infection in Mice Brains
2.2. Characteristics of circRNAs Expressed in Mice Brain by RNA Sequencing
2.3. Identification of circRNAs Expression in RABV Infection
2.4. Expression Correlation of circRNAs and mRNAs
2.5. Functional Enrichment Analysis of Host Genes of Differentially Expressed circRNAs
2.6. Interaction Network of circRNA–miRNA–mRNA
3. Discussion
4. Materials and Methods
4.1. RABV Infection
4.2. RNA Extraction and Quantitative Real-Time PCR
4.3. Immunohistochemical (IHC) Analysis
4.4. RNA Sequencing Analysis
4.5. Functional Enrichment Analysis
4.6. Gene Set Enrichment Analysis (GSEA)
4.7. Integrated Analysis of circRNAs-miRNAs-mRNAs
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oligonucleotide Sequences | 5′–3′ |
---|---|
novel_circ_015068 | TGCATAATCAGCTACGGTGGTG |
TGGAGCCGTGATAGTAAACGC | |
novel_circ_017745 | TTGGTTCTTCAAAATCGCTGCC |
TCTGCCTTTGTATCAACCATCTTG | |
novel_circ_015161 | GCTGTGCAATGGTGAAATTATGG |
CACTGAGCATCACTCACTCCTTT | |
novel_circ_011422 | GCTGTGTGACGTTCAGGAAGAA |
TTCAAGGCCGCCTCTTCTTTG | |
novel_circ_012539 | TTGGAATGTTGGACTCCTTGAGA |
CCTTCATCCAACCAGGCACTT | |
novel_circ_010735 | CCACAGCCTTGCTTGTGAAA |
CTGTTTCCTCTACTGATGGTGGG | |
novel_circ_008143 | ACCCGAAGAAAGATGGATCGG |
CAGGCTCCCAAGATAAGCGA | |
novel_circ_010627 | ACGGAGAGTATGTTGTCGAGGT |
AACCACGGCTCCAAGTCAGT | |
novel_circ_010564 | TGGACCCTGCTAAGAAGTCACC |
TCTGCAATGTCCGGCCTCA | |
novel_circ_026888 | TGCCTCTGTTTTATGCTGCTACC |
TGGTTACGGTCAGTCAGTGGT | |
novel_circ_006420 | AGTCAAAGTCCCACGGCCAC |
TAGTGTTGAAGAGGGAGAGTGGT | |
novel_circ_014267 | TTTCGACGTTTTCAGCGAGACA |
GCCTTCTTCAGACATCATCAGTGG |
circRNAs | Source Gene | Oligonucleotide Sequences (5′–3′) |
---|---|---|
novel_circ_015068 | PD-L1 | CCTGCTGTCACTTGCTACGG |
TCCAGCTCCCGTTCTACAGG | ||
novel_circ_015161 | Pcgf5 | TGCCCTTCTGCTACTGACCA |
GGCAAGCGGAACACTGAGAA | ||
novel_circ_011422 | Parp9 | ACAGGGAAGAGCAAAGGCGA |
GTGGCCTGTTTCGGGTGATG | ||
novel_circ_012539 | - | |
novel_circ_017745 | Serinc3 | AGGAACATCAGCCTCGCTCT |
GGACCGCTCAGGTTCATTGG | ||
novel_circ_010735 | - | |
novel_circ_008143 | Ocln | GGGAAAGCAGGAAAGGGCAA |
CTGACCCAGTCCTCCTCCAG | ||
novel_circ_010627 | Cntn1 | GGGCTGGGCATGACAAAGAA |
TGGGTGTCGGGAAGAAGGTT | ||
novel_circ_010564 | Shank3 | TTCCTCTCTGTGGGTGCCAT |
CAGGGGAGGGGAGTAGCAAA | ||
novel_circ_026888 | Slc20a2 | GGGTTTGGGGCAGAAGAGTG |
TGTTGGAGGCAATCACCACG | ||
novel_circ_006420 | Rtn1 | GGGTTTGGCACATCCCCTAA |
TTCGCTACTCCCAAGCCTGT | ||
novel_circ_014267 | Atp9b | ACAGTTCACGGGCTGGTTTC |
ATGGGGGCATCTCGAAGTCA |
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Zhao, W.; Su, J.; Wang, N.; Zhao, N.; Su, S. Expression Profiling and Bioinformatics Analysis of CircRNA in Mice Brain Infected with Rabies Virus. Int. J. Mol. Sci. 2021, 22, 6537. https://doi.org/10.3390/ijms22126537
Zhao W, Su J, Wang N, Zhao N, Su S. Expression Profiling and Bioinformatics Analysis of CircRNA in Mice Brain Infected with Rabies Virus. International Journal of Molecular Sciences. 2021; 22(12):6537. https://doi.org/10.3390/ijms22126537
Chicago/Turabian StyleZhao, Wen, Jingyin Su, Ningning Wang, Naiyu Zhao, and Shuo Su. 2021. "Expression Profiling and Bioinformatics Analysis of CircRNA in Mice Brain Infected with Rabies Virus" International Journal of Molecular Sciences 22, no. 12: 6537. https://doi.org/10.3390/ijms22126537