CDC2 Is an Important Driver of Vascular Smooth Muscle Cell Proliferation via FOXM1 and PLK1 in Pulmonary Arterial Hypertension
Abstract
:1. Introduction
2. Results
2.1. Expression of CDC2 in HPASMC
2.2. Effect of Timing, Cell Cycle Arrest, and FOXM1/PLK1 Inhibition on Expression of CDC2
2.3. Effectors of CDC2 Expression
2.4. Expression of Other CDKs
2.5. Regulators of CDC2
2.6. Expression of CDC2 in Pulmonary Arteries from Sugen/Hypoxia-Treated Rats
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Cell Culture
4.3. Transfection with siRNA
4.4. Real-Time qPCR
4.5. Western Blot
4.6. Animal Experiments
4.7. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Subject | Code ID | Gender | Age | Germline Mutation |
---|---|---|---|---|
CON1 | C128 | Male | 36 | None |
CON2 | A678 | Male | 39 | None |
CON3 | TRL-CON-4, E352 | Female | 48 | None |
CON4 | Lot#1189 | Female | 17 | None |
CON5 | Lot#1487 | Male | 21 | None |
PAH1 | D355 | Male | 42 | None |
PAH2 | ST012 F281 | Female | 26 | Smad-8 R294X |
PAH3 | CCF005 E037 | Female | 47 | None |
PAH4 | IPAH16 F518 | ? | ? | None |
PAH5 | CC008 | ? | ? | None |
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Pal-Ghosh, R.; Xue, D.; Warburton, R.; Hill, N.; Polgar, P.; Wilson, J.L. CDC2 Is an Important Driver of Vascular Smooth Muscle Cell Proliferation via FOXM1 and PLK1 in Pulmonary Arterial Hypertension. Int. J. Mol. Sci. 2021, 22, 6943. https://doi.org/10.3390/ijms22136943
Pal-Ghosh R, Xue D, Warburton R, Hill N, Polgar P, Wilson JL. CDC2 Is an Important Driver of Vascular Smooth Muscle Cell Proliferation via FOXM1 and PLK1 in Pulmonary Arterial Hypertension. International Journal of Molecular Sciences. 2021; 22(13):6943. https://doi.org/10.3390/ijms22136943
Chicago/Turabian StylePal-Ghosh, Ruma, Danfeng Xue, Rod Warburton, Nicholas Hill, Peter Polgar, and Jamie L. Wilson. 2021. "CDC2 Is an Important Driver of Vascular Smooth Muscle Cell Proliferation via FOXM1 and PLK1 in Pulmonary Arterial Hypertension" International Journal of Molecular Sciences 22, no. 13: 6943. https://doi.org/10.3390/ijms22136943