Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes
Abstract
:1. Introduction
2. Results
2.1. Development of U2af1 Mutant Allele
2.2. Old U2af1 Mutant Mice Show Normal Hematopoiesis and Uneven Splenomegaly
2.3. Monogranulocytic Progenitors Are Increased in U2af1mut/+ Tet2−/− Mutant Mice
2.4. U2af1mut/+ and U2af1mut/+ Tet2−/− Mutant HSPC Show a Defect in Reconstitution Capacity
2.5. Exon Skipping Is the Alternatively Spliced Event More Frequently Observed
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. RT-qPCR
4.3. Western Blotting
4.4. Blood Count Analysis
4.5. Flow Cytometry Analysis
4.6. Histological Analysis
4.7. Cytospin Preparation
4.8. Colony-Forming Unit Assays
4.9. DNA Damage
4.10. LSK FACS-Sorting
4.11. Competitive Repopulation Assay
4.12. Behaviour Tests
4.13. RNA Sequencing Library Construction
4.14. RNA Sequencing Analysis
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Martínez-Valiente, C.; Garcia-Ruiz, C.; Rosón, B.; Liquori, A.; González-Romero, E.; Fernández-González, R.; Gómez-Redondo, I.; Cervera, J.; Gutiérrez-Adán, A.; Sanjuan-Pla, A. Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes. Int. J. Mol. Sci. 2021, 22, 6963. https://doi.org/10.3390/ijms22136963
Martínez-Valiente C, Garcia-Ruiz C, Rosón B, Liquori A, González-Romero E, Fernández-González R, Gómez-Redondo I, Cervera J, Gutiérrez-Adán A, Sanjuan-Pla A. Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes. International Journal of Molecular Sciences. 2021; 22(13):6963. https://doi.org/10.3390/ijms22136963
Chicago/Turabian StyleMartínez-Valiente, Cristina, Cristian Garcia-Ruiz, Beatriz Rosón, Alessandro Liquori, Elisa González-Romero, Raúl Fernández-González, Isabel Gómez-Redondo, José Cervera, Alfonso Gutiérrez-Adán, and Alejandra Sanjuan-Pla. 2021. "Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes" International Journal of Molecular Sciences 22, no. 13: 6963. https://doi.org/10.3390/ijms22136963