Exploring New Potential Anticancer Activities of the G-Quadruplexes Formed by [(GTG2T(G3T)3] and Its Derivatives with an Abasic Site Replacing Single Thymidine
Abstract
:1. Introduction
2. Results and Discussion
2.1. NMR Spectroscopy
2.2. CD Spectroscopy
2.3. Polyacrylamide Gel Electrophoresis (PAGE)
2.4. Nuclease Stability Assay
2.5. Antiproliferative Activity
2.6. Anti-Motility Property
3. Materials and Methods
3.1. Oligonucleotide Synthesis and Purification
3.2. NMR Spectroscopy
3.3. CD Spectroscopy
3.4. Gel Electrophoresis
3.5. Nuclease Stability Assay
3.6. Cell Cultures and Treatments with the ODNs
3.7. MTT Assay
3.8. Wound Healing Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Oligonucleotide | Sequence | Tm (°C) ± 1 |
---|---|---|
INT-B (T30175) | 5′-GTGGTGGGTGGGTGGGT-3′ | 86 |
INT-BS2 | 5′-GSGGTGGGTGGGTGGGT-3′ | 86 |
INT-BS5 | 5′-GTGGSGGGTGGGTGGGT-3′ | 84 |
INT-BS9 | 5′-GTGGTGGGSGGGTGGGT-3′ | 85 |
INT-BS13 | 5′-GTGGTGGGTGGGSGGGT-3′ | 84 |
INT-BS17 | 5′-GTGGTGGGTGGGTGGGS-3′ | 83 |
TT-INT-B | 5′-TTGTGGTGGGTGGGTGGGT-3′ | 84 |
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Virgilio, A.; Benigno, D.; Pecoraro, A.; Russo, A.; Russo, G.; Esposito, V.; Galeone, A. Exploring New Potential Anticancer Activities of the G-Quadruplexes Formed by [(GTG2T(G3T)3] and Its Derivatives with an Abasic Site Replacing Single Thymidine. Int. J. Mol. Sci. 2021, 22, 7040. https://doi.org/10.3390/ijms22137040
Virgilio A, Benigno D, Pecoraro A, Russo A, Russo G, Esposito V, Galeone A. Exploring New Potential Anticancer Activities of the G-Quadruplexes Formed by [(GTG2T(G3T)3] and Its Derivatives with an Abasic Site Replacing Single Thymidine. International Journal of Molecular Sciences. 2021; 22(13):7040. https://doi.org/10.3390/ijms22137040
Chicago/Turabian StyleVirgilio, Antonella, Daniela Benigno, Annalisa Pecoraro, Annapina Russo, Giulia Russo, Veronica Esposito, and Aldo Galeone. 2021. "Exploring New Potential Anticancer Activities of the G-Quadruplexes Formed by [(GTG2T(G3T)3] and Its Derivatives with an Abasic Site Replacing Single Thymidine" International Journal of Molecular Sciences 22, no. 13: 7040. https://doi.org/10.3390/ijms22137040