EVOO Polyphenols Relieve Synergistically Autophagy Dysregulation in a Cellular Model of Alzheimer’s Disease
Abstract
:1. Introduction
2. Results
2.1. OleA/HT Mixtures Trigger Autophagy
2.2. Cells Pre-Treatment with the MIX Prevents Aβ Oligomers Cytotoxicity
2.3. The MIX Favours Aβ1−42 Oligomers Digestion by Autophagy
2.4. The MIX Preserves the Autophagic Flux
2.5. The MIX Affects Mitochondria Functions
2.6. Effects of the MIX on the Cross-Talk between Autophagy and the Ubiquitin Proteasome System
3. Discussion
4. Materials and Methods
4.1. Aβ1–42 Aggregation
4.2. Preparation of Oleuropein Aglycone and Hydroxytyrosol Samples
4.3. Cell Culture
4.4. MTT Assay
4.5. ROS Determination
4.6. Western-Blotting
4.7. Autophagosome Detection
4.8. Mitochondrial Membrane Potential
4.9. Quantification of Active Mitochondria
4.10. Immunofluorescence
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Leri, M.; Bertolini, A.; Stefani, M.; Bucciantini, M. EVOO Polyphenols Relieve Synergistically Autophagy Dysregulation in a Cellular Model of Alzheimer’s Disease. Int. J. Mol. Sci. 2021, 22, 7225. https://doi.org/10.3390/ijms22137225
Leri M, Bertolini A, Stefani M, Bucciantini M. EVOO Polyphenols Relieve Synergistically Autophagy Dysregulation in a Cellular Model of Alzheimer’s Disease. International Journal of Molecular Sciences. 2021; 22(13):7225. https://doi.org/10.3390/ijms22137225
Chicago/Turabian StyleLeri, Manuela, Andrea Bertolini, Massimo Stefani, and Monica Bucciantini. 2021. "EVOO Polyphenols Relieve Synergistically Autophagy Dysregulation in a Cellular Model of Alzheimer’s Disease" International Journal of Molecular Sciences 22, no. 13: 7225. https://doi.org/10.3390/ijms22137225