Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of Flavonoids from C. lanceolata Flowers
2.2. Inhibitory Effect of the Five Natural Compounds Isolated from C. lanceolata Flowers on Nitrite and PGE2 Production
2.3. Inhibitory Effects of the Five Natural Compounds Isolated from C. lanceolate Flowers on Cytokine Production
2.4. Inhibitory Effect of Phenylheptatriyne on iNOS and COX-2 Expression
2.5. Inhibitory Effect of Phenylheptatriyne on NF-κB Translocation
2.6. The Expression of HO-1 using by Phenylhpetatriyne and Inhibitory Effect of Phenylhpetatriyne on Nitrite Production through the regulation of HO-1 Activity
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Materials and Instruments Used for Isolation
4.3. Isolation Procedure of Compounds (1–5) from C. lanceolata Flowers
4.4. Cell Culture and MTT Assay
4.5. Measurement of Nitrite Oxide (NO) Generation
4.6. Prostaglandin (PGE2) Assay
4.7. Interleukin (IL)-6 and Tumor Necrosis Factor (TNF)-α Assay
4.8. Western Blot Analysis and the Extraction of Cytoplasmic and Nuclear Cell Fractions
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lee, H.; Liu, Z.; Yoon, C.-S.; Dong, L.; Ko, W.; Woo, E.-R.; Lee, D.-S. Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells. Int. J. Mol. Sci. 2021, 22, 7482. https://doi.org/10.3390/ijms22147482
Lee H, Liu Z, Yoon C-S, Dong L, Ko W, Woo E-R, Lee D-S. Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells. International Journal of Molecular Sciences. 2021; 22(14):7482. https://doi.org/10.3390/ijms22147482
Chicago/Turabian StyleLee, Hwan, Zhiming Liu, Chi-Su Yoon, Linsha Dong, Wonmin Ko, Eun-Rhan Woo, and Dong-Sung Lee. 2021. "Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells" International Journal of Molecular Sciences 22, no. 14: 7482. https://doi.org/10.3390/ijms22147482