Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species
Abstract
:1. Introduction
2. Results
2.1. DOX Inhibited Fetal GMC Proliferation
2.2. DOX Reduced TRPC6 Channel Expression in Fetal GMCs
2.3. DOX Inhibited TRPC6-Mediated [Ca2+]i Elevation in Fetal GMCs
2.4. DOX Stimulated Mitochondrial ROS Generation in Fetal GMCs
2.5. MitoQ Reversed DOX-Induced Apoptosis in Fetal GMCs
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Primary GMC Culture
4.3. Live-Cell Imaging
4.4. Western Immunoblotting
4.5. Intracellular Ca2+ [Ca2+]i Imaging
4.6. Determination of Mitochondria ROS and Mitophagy Assay
4.7. Antibodies (Catalog Numbers Are in Parentheses) and Chemicals
4.8. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Matthews, A.T.; Soni, H.; Robinson-Freeman, K.E.; John, T.A.; Buddington, R.K.; Adebiyi, A. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. Int. J. Mol. Sci. 2021, 22, 7589. https://doi.org/10.3390/ijms22147589
Matthews AT, Soni H, Robinson-Freeman KE, John TA, Buddington RK, Adebiyi A. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. International Journal of Molecular Sciences. 2021; 22(14):7589. https://doi.org/10.3390/ijms22147589
Chicago/Turabian StyleMatthews, Anberitha T., Hitesh Soni, Katherine E. Robinson-Freeman, Theresa A. John, Randal K. Buddington, and Adebowale Adebiyi. 2021. "Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species" International Journal of Molecular Sciences 22, no. 14: 7589. https://doi.org/10.3390/ijms22147589